The relative abundance of fecal bacterial species belonging to the Firmicutes and Bacteroidetes phyla is related to plasma levels of bile acids in young adults
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AuthorOsuna Prieto, Francisco J.; Xu, Huiwen; Ortiz Álvarez, Lourdes; Jurado Fasoli, Lucas; Rubio‑Lopez, Jose; Plaza Díaz, Julio; Gil Hernández, Ángel; Ruiz, Jonatan R.; Martínez Téllez, Borja Manuel
7-α-DehydroxylasesBile salt hydrolasesGut microbiotaMicrobiome
Osuna-Prieto, F.J., Xu, H., Ortiz-Alvarez, L. et al. The relative abundance of fecal bacterial species belonging to the Firmicutes and Bacteroidetes phyla is related to plasma levels of bile acids in young adults. Metabolomics 19, 54 (2023). [https://doi.org/10.1007/s11306-023-02016-8]
SponsorshipUniversidad de Granada/CBUA; Spanish Ministry of Economy and Competitiveness via Fondo de Investigacion Sanitaria del Instituto de Salud Carlos III PI13/01393; Retos de la Sociedad DEP2016-79512-R; European Commission; Spanish Government FPU16/05159, FPU16/02828, FPU17/01523, FPU19/01609; Fundacion Iberoamericana de Nutricion (FINUT); University of Granada Plan Propio de Investigacion 2016-Excellence actions: Unit of Excellence on Exercise and Health (UCEES); AstraZeneca; Junta de Andalucia P18-RT-4455; China Scholarship Council 201707060012; Ministerio de Universidades y la Union Europea -Next Generation EU RR_C_2021_04; MIRACUM; Federal Ministry of Education & Research (BMBF) FKZ 01ZZ1801H; University of Granada, Spain; European Commission Joint Research Centre ZS/2018/11/95324
Background Gut bacteria play a crucial role in the metabolism of bile acids (BA). Whether an association exists between the fecal microbiota composition and circulating BA levels in humans is poorly understood. Here, we investigated the relationship between fecal microbiota diversity and composition with plasma levels of BA in young adults. Methods Fecal microbiota diversity/composition was analyzed with 16S rRNA sequencing in 80 young adults (74% women; 21.9 +/- 2.2 years old). Plasma levels of BA were measured using liquid chromatography-tandem mass spectrometry. PERMANOVA and Spearman correlation analyses were used to investigate the association between fecal microbiota parameters and plasma levels of BA. Results Fecal microbiota beta (P = 0.025) and alpha diversity indexes of evenness (rho = 0.237, P = 0.033), Shannon (rho = 0.313, P = 0.004), and inverse Simpson (rho = 0.283, P = 0.010) were positively associated with plasma levels of the secondary BA glycolithocholic acid (GLCA). The relative abundance of genera belonging to the Firmicutes and Bacteroidetes phyla was positively correlated with plasma levels of GLCA (all rho = 0.225, P = 0.049). However, the relative abundance of species from Firmicutes and Bacteroidetes phyla were negatively correlated with plasma levels of primary and secondary BA (all rho = - 0.220, P = 0.045), except for the relative abundance of Bacteroides vulgatus, Alistipes onderdonkii, and Bacteroides xylanisolvens species (Bacteroidetes phylum) that were positively correlated with the plasma levels of GLCA. Conclusions The relative abundance of specific fecal bacteria species is associated with plasma levels of BA in young adults. However, further investigations are required to validate whether the composition of the gut microbiota can regulate the plasma concentrations of BA in humans.