A comparison of five DNA extraction methods from degraded human skeletal remains
Metadatos
Afficher la notice complèteAuteur
Haarkötter Cardoso, Christian; Gálvez Escolano, Xiomara; Vinueza Espinosa, Diana C.; Medina Lozano, María Isabel; Sáiz Guinaldo, María; Lorente Acosta, José Antonio; Álvarez Merino, Juan CarlosEditorial
Elsevier
Materia
Degraded DNA DNA extraction Human remains Skeletal remains STR typing
Date
2023-05-15Referencia bibliográfica
C. Haarkötter, X. Gálvez, D.C. Vinueza-Espinosa et al. A comparison of five DNA extraction methods from degraded human skeletal remains. Forensic Science International 348 (2023) 111730[https://doi.org/10.1016/j.forsciint.2023.111730]
Résumé
Extracting DNA from degraded human remains poses a challenge for any forensic genetics laboratory, as it
requires efficient high-throughput methods. While little research has compared different techniques, silica
in suspension has been identified in the literature as the best method for recovering small fragments, which
are often present in these types of samples. In this study, we tested five DNA extraction protocols on 25
different degraded skeletal remains. Including the humerus, ulna, tibia, femur, and petrous bone. The five
protocols were organic extraction by phenol/chloroform/isoamyl alcohol, silica in suspension, High Pure
Nucleic Acid Large Volume silica columns (Roche), InnoXtract™ Bone (InnoGenomics), and PrepFiler™ BTA
with AutoMate™ Express robot (ThermoFisher). We analysed five DNA quantification parameters (small
human target quantity, large human target quantity, human male target quantity, degradation index, and
internal PCR control threshold), and five DNA profile parameters (number of alleles with peak height higher
than analytic and stochastic threshold, average relative fluorescence units (RFU), heterozygous balance, and
number of reportable loci) were analysed. Our results suggest that organic extraction by phenol/chloroform/
isoamyl alcohol was the best performing method in terms of both quantification and DNA profile
results. However, Roche silica columns were found to be the most efficient method.