Mostrar el registro sencillo del ítem

dc.contributor.authorMartín-Sierra, Carmen
dc.contributor.authorEscobedo, Pablo
dc.contributor.authorRuiz García, Isidoro 
dc.contributor.authorErenas, Miguel M.
dc.contributor.authorSánchez Martín, Rosario María 
dc.contributor.authorCapitán Vallvey, Luis Fermín 
dc.contributor.authorPalma López, Alberto José 
dc.contributor.authorDíaz Mochón, Juan José 
dc.date.accessioned2023-06-14T10:47:44Z
dc.date.available2023-06-14T10:47:44Z
dc.date.issued2023-06
dc.identifier.citationC. Martín-Sierra et al. SARS-CoV-2 viral RNA detection using the novel CoVradar device associated with the CoVreader smartphone app. Biosensors and Bioelectronics 230 (2023) 115268. [https://doi.org/10.1016/j.bios.2023.115268]es_ES
dc.identifier.urihttps://hdl.handle.net/10481/82456
dc.descriptionSupplementary data to this article can be found online at https://doi. org/10.1016/j.bios.2023.115268es_ES
dc.description.abstractThe COVID-19 pandemic has highlighted the need for innovative approaches to its diagnosis. Here we present CoVradar, a novel and simple colorimetric method that combines nucleic acid analysis with dynamic chemical labeling (DCL) technology and the Spin-Tube device to detect SARS-CoV-2 RNA in saliva samples. The assay includes a fragmentation step to increase the number of RNA templates for analysis, using abasic peptide nucleic acid probes (DGL probes) immobilized to nylon membranes in a specific dot pattern to capture RNA fragments. Duplexes are formed by labeling complementary RNA fragments with biotinylated SMART bases, which act as templates for DCL. Signals are generated by recognizing biotin with streptavidin alkaline phosphatase and incubating with a chromogenic substrate to produce a blue precipitate. CoVradar results are analysed by CoVreader, a smartphone-based image processing system that can display and interpret the blotch pattern. CoVradar and CoVreader provide a unique molecular assay capable of detecting SARS-CoV-2 viral RNA without the need for extraction, preamplification, or pre-labeling steps, offering advantages in terms of time (similar to 3 h/test), cost (similar to epsilon 1/test manufacturing cost) and simplicity (does not require large equipment). This solution is also promising for developing assays for other infectious diseases.es_ES
dc.description.sponsorshipFEDER/Junta de Andalucia-Consejeria de Economia y Conocimiento CV20-77741, A-FQM-760-UGR20, PID 2019-110987RB-I00, PID 2019-103938RB-I00es_ES
dc.description.sponsorshipSpanish MCIN/AEI P18-RT-2961, P18-TP-4160es_ES
dc.description.sponsorshipFEDER/Junta de Andalucia-Consejeria de Salud y Familias PIP-0232-2021es_ES
dc.description.sponsorshipEuropean Commissiones_ES
dc.description.sponsorshipMCIN/AEI PTQ 2020-011388, IJC 2020-043307-Ies_ES
dc.description.sponsorshipEuropean Union NextGenerationEU/PRTR PTQ 2020-011388, IJC 2020-043307-Ies_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectSevere Acute Respiratory Syndromees_ES
dc.subjectCoronavirus 2 (SARS-CoV-2)es_ES
dc.subjectCoronavirus disease 19 (COVID-19)es_ES
dc.subjectColourimetric assayes_ES
dc.subjectDynamic chemistry labelling (DCL)es_ES
dc.subjectImage processing system (IPS)es_ES
dc.subjectCOVID-19es_ES
dc.titleSARS-CoV-2 viral RNA detection using the novel CoVradar device associated with the CoVreader smartphone appes_ES
dc.typejournal articlees_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/NextGenerationEU/PTQ 2020-011388/IJC 2020-043307-Ies_ES
dc.rights.accessRightsopen accesses_ES
dc.identifier.doi10.1016/j.bios.2023.115268
dc.type.hasVersionVoRes_ES


Ficheros en el ítem

[PDF]

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Excepto si se señala otra cosa, la licencia del ítem se describe como Attribution-NonCommercial-NoDerivatives 4.0 Internacional