Characterization and Molecular Modelling of Non-Antibiotic Nanohybrids for Wound Healing Purposes
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AuthorValentino, Caterina; Martínez Rodríguez, Tomás; Hernández Benavides, Pablo José; Arrebola Vargas, Francisco Jesús; Paredes Martínez, José Manuel; Sainz Díaz, Claro Ignacio; Medina Pérez, María Del Mar; Aguzzi, Carola
BentoniteHalloysiteChlorhexidineSpray dryingMolecular modellingWound healingChronic woundsBiocompatibilityAntimicrobial propertiesAntibiotic resistance
Valentino, C.; Martínez Rodríguez, T.; Borrego-Sánchez, A.; Hernández Benavides, P.; Arrebola Vargas, F.; Paredes, J.M.; Rossi, S.; Sainz Díaz, C.I.; Sandri, G.; Grisoli, P.; et al. Characterization and Molecular Modelling of Non-Antibiotic Nanohybrids for Wound Healing Purposes. Pharmaceutics 2023, 15, 1140. [https://doi.org/10.3390/pharmaceutics15041140]
SponsorshipAndalusian PID2020-112737RB-I00 MCIN/AEI/10.13039/501100011033; P18-RT-3786
The healing process of chronic wounds continues to be a current clinical challenge, wors- ened by the risk of microbial infections and bacterial resistance to the most frequent antibiotics. In this work, non-antibiotic nanohybrids based on chlorhexidine dihydrochloride and clay minerals have been developed in order to design advanced therapeutic systems aimed to enhance wound healing in chronic lesions. To prepare the nanohybrids, two methodologies have been compared: the intercala- tion solution procedure and the spray-drying technique, the latter as a one-step process able to reduce preparation times. Nanohybrids were then fully studied by solid state characterization techniques. Computational calculations were also performed to assess the interactions between the drug and the clays at the molecular level. In vitro human fibroblast biocompatibility and antimicrobial activity against Staphylococcus aureus and Pseudomonas aeruginosa were assessed to check biocompatibility and potential microbicidal effects of the obtained nanomaterials. The results demonstrated the effective organic/inorganic character of the nanohybrids with homogeneous drug distribution into the clayey structures, which had been confirmed by classical mechanics calculations. Good biocompatibility and microbicidal effects were also observed, especially for the spray-dried nanohybrids. It was suggested that it could be due to a greater contact area with target cells and bacterial suspensions.