Identification of Candidate Genes Involved in the Determinism of Pollen Grain Aperture Morphology by Comparative Transcriptome Analysis in Papaveraceae
Metadatos
Mostrar el registro completo del ítemEditorial
MDPI
Materia
Genetic determinism Papaveraceae Pollen aperture Transcriptome analysis RNA-seq
Fecha
2023-04-06Referencia bibliográfica
Mazuecos-Aguilera, I.; Suárez-Santiago, V.N. Identification of Candidate Genes Involved in the Determinism of Pollen Grain Aperture Morphology by Comparative Transcriptome Analysis in Papaveraceae. Plants 2023, 12, 1570. [https://doi.org/10.3390/plants12071570]
Patrocinador
Spanish Government CGL2015-70290-P; Predoctoral grant (F.P.I. program) from the Spanish GovernmentResumen
In the last decade, certain genes involved in pollen aperture formation have been discov-
ered. However, those involved in pollen aperture shape remain largely unknown. In Arabidopsis,
the interaction during the tetrad development stage of one member of the ELMOD protein fam-
ily, ELMOD_E, with two others, MCR/ELMOD_B and ELMOD_A, can change the morphology of
apertures from colpus (elongated) to pore (round). Here, comparative transcriptome analysis is
used to identify candidate genes involved in the determination of pollen aperture morphology in
Papaveraceae (order Ranunculales). Furthermore, the role of ELMOD genes in the genetic determin-
ism of aperture shape was tested by comparative analysis of their expression levels using RNA-seq
data and RT-qPCR. Two pairs of species belonging to two different subfamilies were used. Within
each pair, one species has colpate pollen and the other porate (Fumarioideae—Dactylicapnos torulosa,
6-colpate, and Fumaria bracteosa, pantoporate; Papaveroideae—Eschsholzia californica, 5–7 colpate, and
Roemeria refracta, 6-porate). The transcriptomes were obtained at the tetrad stage of pollen develop-
ment. A total of 531 DEGs were found between the colpate and porate pollen species groups. The
results from RNA-seq and RT-qPCR indicate that pollen aperture shape is not determined by the
relative expression levels of ELMOD family genes in Papaveraceae. However, genes related to callose
wall formation or cytoskeleton organisation were found, these processes being involved in pollen
aperture formation. In addition, transcriptomes from anthers with pollen during the tetrad stage of
three species (D. torulosa, R. refracta, and F. bracteosa) were obtained for the first time. These data will
be available for further studies in the field of floral evolution and development.