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Dexamethasone and doxycycline functionalized nanoparticles enhance osteogenic properties of titanium surfaces
dc.contributor.author | Toledano Osorio, Manuel | |
dc.contributor.author | Luna Bertos, María Elvira De | |
dc.contributor.author | Toledano Pérez, Manuel | |
dc.contributor.author | Manzano Moreno, Francisco Javier | |
dc.contributor.author | Costela Ruiz, Víctor Javier | |
dc.contributor.author | Ruiz Rodríguez, Concepción | |
dc.contributor.author | Gil, Javier | |
dc.contributor.author | Osorio Ruiz, Raquel | |
dc.date.accessioned | 2023-05-16T06:46:07Z | |
dc.date.available | 2023-05-16T06:46:07Z | |
dc.date.issued | 2023-05-10 | |
dc.identifier.citation | M. Toledano-Osorio, E. de Luna-Bertos, M. Toledano et al., Dexamethasone and doxycycline functionalized nanoparticles enhance osteogenic properties of titanium surfaces, Dental Materials, [https://doi.org/10.1016/j.dental.2023.05.004] | es_ES |
dc.identifier.uri | https://hdl.handle.net/10481/81546 | |
dc.description.abstract | Objectives: To evaluate the effect of doxycycline and dexamethasone doped nanoparticles covering titanium surfaces, on osteoblasts proliferation and differentiation. Methods: Doxycycline and dexamethasone doped polymeric nanoparticles were applied on titanium discs (Ti- DoxNPs and Ti-DexNPs). Undoped NPs and uncovered Ti discs were used as control. Human MG-63 osteoblast- like cells were cultured. Osteoblasts proliferation was tested by MTT assay. Alkaline phosphatase activity was analyzed. Differentiation gene expression was assessed by real-time quantitative polymerase chain reaction. Scanning Electron Microscopy was performed to assess osteoblasts morphology. Mean comparisons were con- ducted by ANOVA and Wilcoxon or Tukey tests (p < 0.05). Results: No differences in osteoblasts proliferation were found. Osteoblasts grown on Ti-DoxNPs significantly increased alkaline phosphatase activity. Doxycycline and dexamethasone nanoparticles produced an over-ex- pression of the main osteogenic proliferative genes (TGF-β1, TGF-βR1 and TGF-βR2). The expression of Runx-2 was up-regulated. The osteogenic proteins (AP, OSX and OPG) were also overexpressed on osteoblasts cultured on Ti-DoxNPs and Ti-DexNPs. The OPG/RANKL ratio was the highest when DoxNPs were present (75-fold in- crease with respect to the control group). DexNPs also produced a significantly higher OPG/RANKL ratio with respect to the control (20 times higher). Osteoblasts grown on titanium discs were mainly flat and polygonal in shape, with inter-cellular connections. In contrast, osteoblasts cultured on Ti-DoxNPs or Ti-DexNPs were found to be spindle-shaped and had abundant secretions on their surfaces. Significance: DoxNPs and DexNPs were able to stimulate osteoblasts differentiation when applied on titanium surfaces, being considered potential inducers of osteogenic environment when performing regenerative proce- dures around titanium dental implants. | es_ES |
dc.description.sponsorship | Grant PID2020–114694RB-I00 funded by MCIN/AEI 10.13039/501100011033 | es_ES |
dc.description.sponsorship | FPU of Ministry of Universities grant FPU20/0045 | es_ES |
dc.description.sponsorship | Klockner S.A. for financial support and for providing the titanium discs | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | es_ES |
dc.subject | Titanium | es_ES |
dc.subject | Nanoparticles | es_ES |
dc.subject | Osteoblasts | es_ES |
dc.subject | Osteogenic | es_ES |
dc.subject | Dexamethasone | es_ES |
dc.subject | Doxycycline | es_ES |
dc.subject | Polymers | es_ES |
dc.title | Dexamethasone and doxycycline functionalized nanoparticles enhance osteogenic properties of titanium surfaces | es_ES |
dc.type | journal article | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.identifier.doi | 10.1016/j.dental.2023.05.004 | |
dc.type.hasVersion | VoR | es_ES |