Antioxidant and Chemopreventive Activity of Protein Hydrolysates from Raw and Germinated Flour of Legumes with Commercial Interest in Colorectal Cancer
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AuthorFuel, Marco; Mesas Hernández, Cristina; Martínez Martínez, Rosario; Ortiz Quesada, Raúl; Quiñonero Muñoz, Francisco José; Kapravelou, Garyfallia; Lozano Melero, Aída; Perazzoli, Gloria; Prados Salazar, José Carlos; Porres Foulquie, Jesús María; Melguizo Alonso, Consolación
FabaceaeAntioxidantChemopreventionFunctional extractsColon cancer
Fuel, M... [et al.]. Antioxidant and Chemopreventive Activity of Protein Hydrolysates from Raw and Germinated Flour of Legumes with Commercial Interest in Colorectal Cancer. Antioxidants 2022, 11, 2421. [https://doi.org/10.3390/antiox11122421]
SponsorshipSpanish Ministry of Science, Innovation and Universities, European Union PTQ-17-09172, RTC-2017-6540-1, RTI2018-100934-BI00 and RTC2019-006870-1; Junta de Andalucía P18-TP-1420; FEDER; AGR145 and CTS-107 (Andalusian Government)
Legumes are a highly nutritious source of plant protein, fiber, minerals and vitamins. However, they also contain several bioactive compounds with significant potential benefits for human health. The objectives of this study were to evaluate the antioxidant, antitumor and chemopreventive activity of functional extracts from legumes using raw and germinated flours of six legume species of commercial interest. The methodology carried out consisted on the development of protein hydrolysates, assessment of their antioxidant capacity and in vitro tests on T84, HCT15 and SW480 colorectal cancer (CRC) cell lines. Our results showed a high antitumor activity of protein hydrolysate from M. sativa. Likewise, when combined with 5-Fluorouracile (5-Fu), there was a synergistic effect using extract concentrations from 50 to 175 g/mL and 5-Fu concentrations from 1.5 to 5 M. Similarly, the induction effect on detoxifying enzymes by the extracts of M. sativa, germinated V. faba Baraca LVzt1 and V. narbonensis, which produced a higher induction rate than the positive control sulforaphane (10 M), should be highlighted. Therefore, incorporating these enzymes into the diet could provide nutritional effects, as well as play an effective role in cancer chemoprevention and therapy.