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dc.contributor.authorAguinaga Casañas, María Aránzazu
dc.contributor.authorMut Salud, Nuria
dc.contributor.authorFalcón Piñeiro, Ana
dc.contributor.authorAlcaraz Martínez, Ángela
dc.contributor.authorGuillamón, Enrique
dc.contributor.authorBaños, Alberto
dc.identifier.citationAguinaga-Casañas, M.A... [et al.]. In Vitro Antiparasitic Activity of Propyl-Propane- Thiosulfinate (PTS) and Propyl-Propane-Thiosulfonate (PTSO) from Allium cepa against Eimeria acervulina Sporozoites. Microorganisms 2022, 10, 2040. []es_ES
dc.description.abstractAmong the alternatives to control avian coccidiosis, alliaceous extracts stand out due to their functional properties. Despite this, most of the references are focused just on garlic. In this study, we analyze the in vitro effects of propyl-propane thiosulfinate (PTS) and propyl-propane thiosulfonate (PTSO), two organosulfur compounds from onion, on MDBK cells infected with sporozoites of Eimeria acervulina. To this aim, two different experiments were performed. In the first experiment, sporozoites were previously incubated for 1 h at 1, 5 and 10 mu g/mL of PTS or PTSO and added to MDBK cells. In the second experiment, MDBK cells were first incubated for 24 h at different concentrations of PTS or PTSO and then infected with E. acervulina sporozoites. Then, 24 h after inoculation, the presence of E. acervulina was quantified by qPCR. MDBK viability was measured at 72 h post-infection. Sporozoites incubated at 10 mu g/mL of PTS and PTSO inhibited the capability to penetrate the cells up to 75.2% +/- 6.44 and 71.7% +/- 6.03, respectively. The incubation of MDBK with each compound resulted in a preventive effect against sporozoite invasion at 1 mu g/mL of PTS and 1 and 10 mu g/mL of PTSO. Cells incubated with PTSO obtained similar viability percentages to uninfected cells. These results suggest that the use of PTS and PTSO is a promising alternative to coccidiosis treatment, although further in vivo studies need to be performed.es_ES
dc.description.sponsorshipCentre for the Development of Industrial Technology in Spain (CDTI) IDI-20220251es_ES
dc.rightsAtribución 4.0 Internacional*
dc.titleIn Vitro Antiparasitic Activity of Propyl-Propane-Thiosulfinate (PTS) and Propyl-Propane-Thiosulfonate (PTSO) from Allium cepa against Eimeria acervulina Sporozoiteses_ES

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