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Bifidobacterium longum subsp. infantis CECT 7210 Reduces Inflammatory Cytokine Secretion in Caco-2 Cells Cultured in the Presence of Escherichia coli CECT 515
dc.contributor.author | Álvarez Mercado, Ana Isabel | |
dc.contributor.author | Plaza Díaz, Julio | |
dc.contributor.author | Gil Hernández, Ángel | |
dc.contributor.author | Fontana Gallego, Luis | |
dc.date.accessioned | 2022-10-17T07:26:30Z | |
dc.date.available | 2022-10-17T07:26:30Z | |
dc.date.issued | 2022-09-16 | |
dc.identifier.citation | Álvarez-Mercado, A.I... [et al.]. Bifidobacterium longum subsp. infantis CECT 7210 Reduces Inflammatory Cytokine Secretion in Caco-2 Cells Cultured in the Presence of Escherichia coli CECT 515. Int. J. Mol. Sci. 2022, 23, 10813. [https://doi.org/10.3390/ijms231810813] | es_ES |
dc.identifier.uri | https://hdl.handle.net/10481/77343 | |
dc.description.abstract | Previous works have described the activity of Bifidobacterium longum subsp. infantis CECT 7210 (also commercially named B. infantis IM-1®) against rotavirus in mice and intestinal pathogens in piglets, as well as its diarrhea-reducing effect on healthy term infants. In the present work, we focused on the intestinal immunomodulatory effects of B. infantis IM-1® and for this purpose we used the epithelial cell line isolated from colorectal adenocarcinoma Caco-2 and a co-culture system of human dendritic cells (DCs) from peripheral blood together with Caco-2 cells. Single Caco-2 cultures and Caco-2: DC co-cultures were incubated with B. infantis IM-1® or its supernatant either in the presence or absence of Escherichia coli CECT 515. The B. infantis IM-1® supernatant exerted a protective effect against the cytotoxicity caused by Escherichia coli CECT 515 on single cultures of Caco-2 cells as viability reached the values of untreated cells. B. infantis IM-1® and its supernatant also decreased the secretion of pro-inflammatory cytokines by Caco-2 cells and the co-cultures incubated in the presence of E. coli CECT 515, with the response being more modest in the latter, which suggests that DCs modulate the activity of Caco-2 cells. Overall, the results obtained point to the immunomodulatory activity of this probiotic strain, which might underlie its previously reported beneficial effects. | es_ES |
dc.description.sponsorship | Laboratorios Ordesa, S. L. | es_ES |
dc.description.sponsorship | Center for the Development of Industrial Technology (CDTI) of the Spanish Ministry of Science and Innovation (TOLERA project) 20170873 | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | B. infantis IM-1® | es_ES |
dc.subject | Bifidobacterium longum subsp. infantis CECT 7210 | es_ES |
dc.subject | Caco-2 cells | es_ES |
dc.subject | Co-cultures | es_ES |
dc.subject | Cytokines | es_ES |
dc.subject | Dendritic cells | es_ES |
dc.subject | Escherichia coli | es_ES |
dc.subject | Probiotics | es_ES |
dc.title | Bifidobacterium longum subsp. infantis CECT 7210 Reduces Inflammatory Cytokine Secretion in Caco-2 Cells Cultured in the Presence of Escherichia coli CECT 515 | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.doi | 10.3390/ijms231810813 | |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |