Role of c-miR-21, c-miR-126, Redox Status, and Inflammatory Conditions as Potential Predictors of Vascular Damage in T2DM Patients
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López Armas, Gabriela C.; Guerra Librero Rite, Ana; Florido Ruiz, Javier; Escames Rosa, Germaine; Acuña Castroviejo, Darío; Rusanova Rusanova, IrynaEditorial
MDPI
Materia
Type 2 diabetes mellitus Vascular complications Circulating miR-21 miR-126 IL-6 Oxidative stress markers
Date
2022-08-27Referencia bibliográfica
López-Armas, G.C... [et al.]. Role of c-miR-21, c-miR-126, Redox Status, and Inflammatory Conditions as Potential Predictors of Vascular Damage in T2DM Patients. Antioxidants 2022, 11, 1675. [https://doi.org/10.3390/antiox11091675]
Sponsorship
University of Granada; Eugenio Rodriguez Pascual Foundation ERP-2021; CIBERfes (ISCIII, Spain) CB16-10-00238Abstract
The development of type 2 diabetes mellitus (T2DM) vascular complications (VCs) is
associated with oxidative stress and chronic inflammation and can result in endothelial dysfunctions.
Circulating microRNAs play an important role in epigenetic regulation of the etiology of T2DM. We
studied 30 healthy volunteers, 26 T2DM patients with no complications, and 26 T2DM patients with
VCs, to look for new biomarkers indicating a risk of developing VCs in T2DM patients. Peripheral
blood samples were used to determine redox state, by measuring the endogenous antioxidant
defense system (superoxide dismutase, SOD; catalase, CAT; glutathione reductase, GRd; glutathione
peroxidase, GPx; and glucose-6-phosphate dehydrogenase, G6DP) and markers of oxidative damage
(advanced oxidation protein products, AOPP; lipid peroxidation, LPO). Additionally, inflammatory
marker levels (IL-1, IL-6, IL-18, and TNF- ), c-miR-21, and c-miR-126 expression were analyzed.
T2DM patients showed the highest oxidative damage with increased GSSG/GSH ratios, LPO, and
AOPP levels. In both diabetic groups, we found that diminished SOD activity was accompanied by
increased CAT and decreased GRd and G6PD activities. Diabetic patients presented with increased
relative expression of c-miR-21 and decreased relative expression of c-miR-126. Overall, c-miR-21,
SOD, CAT, and IL-6 had high predictive values for diabetes diagnoses. Finally, our data demonstrated
that IL-6 exhibited predictive value for VC development in the studied population. Moreover, c-miR-
21 and c-miR-126, along with GPx and AOPP levels, should be considered possible markers for VC
development in future studies.