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dc.contributor.authorMorell, María
dc.contributor.authorVarela, Nieves
dc.contributor.authorCastillejo López, Casimiro
dc.contributor.authorCoppard, Céline
dc.contributor.authorLuque, María José
dc.contributor.authorMartín Morales, Natividad 
dc.contributor.authorPérez Cózar, Francisco
dc.contributor.authorGómez Hernández, Gonzalo
dc.contributor.authorO'Valle Ravassa, Francisco Javier 
dc.contributor.authorAlarcón Riquelme, Marta Eugenia 
dc.contributor.authorMarañón Lizana, Concepción
dc.identifier.citationMaría Morell... [et al.]. SIDT1 plays a key role in type I IFN responses to nucleic acids in plasmacytoid dendritic cells and mediates the pathogenesis of an imiquimod-induced psoriasis model, eBioMedicine, Volume 76, 2022, 103808, ISSN 2352-3964, []es_ES
dc.descriptionThis work was supported by the Consejería de Salud y Familias de la Junta de Andalucía (PIER_S1149 and C2_S0050) and Instituto de Salud Carlos III (PI18/00082 and PI21/01151), partly supported by European FEDER funds, and prior funding to MEAR from the Alliance for Lupus Research and the Swedish Research Council.es_ES
dc.description.abstractSummary Background Type I IFN (IFN-I) is a family of cytokines involved in the pathogenesis of autoimmune and autoinflammatory diseases such as psoriasis. SIDT1 is an ER-resident protein expressed in the lymphoid lineage, and involved in anti-viral IFN-I responses in vivo, through an unclear mechanism. Herein we have dissected the role of SIDT1 in the natural IFN-producing cells, the plasmacytoid dendritic cells (pDC). Methods The function of SIDT1 in pDC was determined by silencing its expression in human primary pDC and GEN2.2 cell line. SIDT1 role in vivo was assessed using the imiquimod-induced psoriasis model in the SIDT1-deficient mice (sidt1-/-). Findings Silencing of SIDT1 in GEN2.2 led to a blockade of the IFN-I response after stimulation of TLR7 and TLR9, without affecting the pro-inflammatory responses or upregulation of maturation markers. We found that SIDT1 migrates from the ER to the endosomal and lysosomal compartments together with TLR9 after CpG stimulation, participating in the access of the TLR9-CpG complex to lysosome-related vesicles, and therefore mediating the activation of TBK1 and the nuclear migration of IRF7, but not of NF -KB. sidt1-/- mice showed a significant decrease in severity parameters of the imiquimod-induced acute psoriasis-like model, associated with a decrease in the production of IFN-I and IFN-dependent chemokines. Interpretation Our findings indicate that SIDT1 is at the cross-road between the IFN-I and the proinflammatory pathways and constitutes a promising drug target for psoriasis and other diseases mediated by IFN-I responses.es_ES
dc.description.sponsorshipConsejería de Salud y Familias de la Junta de Andalucía (PIER_S1149 and C2_S0050)es_ES
dc.description.sponsorshipInstituto de Salud Carlos III (PI18/00082 and PI21/01151)es_ES
dc.description.sponsorshipEuropean FEDER fundses_ES
dc.description.sponsorshipAlliance for Lupus Research and the Swedish Research Counciles_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.subjectProinflammatory cytokineses_ES
dc.subjectPsoriasis es_ES
dc.titleSIDT1 plays a key role in type I IFN responses to nucleic acids in plasmacytoid dendritic cells and mediates the pathogenesis of an imiquimod-induced psoriasis modeles_ES

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Atribución-NoComercial-SinDerivadas 3.0 España
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España