Pseudomonas aeruginosa PAO1 Is Attracted to Bovine Bile in a Novel, Cystic Fibrosis-Derived Bronchial Epithelial Cell Model
Metadatos
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MDPI
Materia
Chemotaxis Bile Pseudomonas aeruginosa Cystic fibrosis Bronchial epithelial cells
Date
2022-03-26Referencia bibliográfica
Behroozian, S... [et al.]. Pseudomonas aeruginosa PAO1 Is Attracted to Bovine Bile in a Novel, Cystic Fibrosis-Derived Bronchial Epithelial Cell Model. Microorganisms 2022, 10, 716. [https://doi.org/10.3390/microorganisms10040716]
Patrocinador
US Cystic Fibrosis Foundation HILL17P0 United States Department of Health & Human Services National Institutes of Health (NIH) - USA; CF Foundation STANTO19R0 R01 HL151385-01ARésumé
Cystic fibrosis (CF) is a life-threatening, inherited, multi-organ disease that renders patients
susceptible throughout their lives to chronic and ultimately deteriorating protracted pulmonary
infections. Those infections are dominated in adulthood by the opportunistic pathogen, Pseudomonas
aeruginosa (Pa). As with other advancing respiratory illnesses, people with CF (pwCF) also frequently
suffer from gastroesophageal reflux disease (GERD), including bile aspiration into the lung. GERD is
a major co-morbidity factor in pwCF, with a reported prevalence of 35–81% in affected individuals.
Bile is associated with the early acquisition of Pa in CF patients and in vitro studies show that it
causes Pa to adopt a chronic lifestyle. We hypothesized that Pa is chemoattracted to bile in the
lung environment. To evaluate, we developed a novel chemotaxis experimental system mimicking
the lung environment using CF-derived bronchial epithelial (CFBE) cells which allowed for the
evaluation of Pa (strain PAO1) chemotaxis in a physiological scenario superior to the standard in vitro
systems. We performed qualitative and quantitative chemotaxis tests using this new experimental
system, and microcapillary assays to demonstrate that bovine bile is a chemoattractant for Pa and is
positively correlated with bile concentration. These results further buttress the hypothesis that bile
likely contributes to the colonization and pathogenesis of Pa in the lung, particularly in pwCF.