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dc.contributor.authorPeñas Fuentes, Juan Luis
dc.contributor.authorRufino Palomares, Eva 
dc.contributor.authorPérez Jiménez, Amalia 
dc.contributor.authorReyes Zurita, Fernando Jesús 
dc.contributor.authorLupiáñez Cara, José Antonio
dc.identifier.citationPeñas-Fuentes, JL... [et al.]. Effects of Erythrodiol on the Antioxidant Response and Proteome of HepG2 Cells. Antioxidants 2022, 11, 73. []es_ES
dc.descriptionThis research was funded by the University of Jaén (Plan Propio de Investigación, grant number UJA2014/07/13) and by Junta de Andalucía (Plan Andaluz de Investigación, Junta de Andalucía, Spain), grant BIO-341 “Enzymes and Metabolism”.es_ES
dc.description.abstractErythrodiol (EO) is a pentacyclic triterpenic alcohol found in olive tree leaves and olive oil, and it has important effects on the health properties and quality of olive oil. In this study, we characterized the cytotoxic effects of EO on human hepatocarcinoma (HepG2) cells by studying changes in cell viability, reactive oxygen species (ROS) production, antioxidant defense systems, and the proteome. The results reveal that EO markedly decreased HepG2 cell viability without changing ROS levels. The concentrations of glutathione and NADPH were significantly reduced, with selective changes in the activity of several antioxidant enzymes: glutathione peroxidase, glutathione reductase, glucose 6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase. Proteomic data reveal that EO led to the complete elimination or decreased abundance of 41 and 3 proteins, respectively, and the abundance of 29 proteins increased. The results of functional enrichment analysis show that important metabolic processes and the nuclear transport of mature mRNA were impaired, whereas AMP biosynthesis and cell cycle G2/M phase transition were induced. The transcription factors and miRNAs involved in this response were also identified. These potent antiproliferative effects make EO a good candidate for the further analysis of its hepatic antitumor effects in in vivo studies.es_ES
dc.description.sponsorshipUniversity of Jaén (Plan Propio de Investigación, grant number UJA2014/07/13)es_ES
dc.description.sponsorshipJunta de Andalucía (Plan Andaluz de Investigación, Junta de Andalucía, Spain), grant BIO-341es_ES
dc.rightsAtribución 3.0 España*
dc.subjectAntioxidant enzymeses_ES
dc.titleEffects of Erythrodiol on the Antioxidant Response and Proteome of HepG2 Cellses_ES

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Atribución 3.0 España
Except where otherwise noted, this item's license is described as Atribución 3.0 España