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dc.contributor.authorMolina Morales, Nerea 
dc.contributor.authorPlaza Díaz, Julio 
dc.contributor.authorSola Leyva, Alberto 
dc.contributor.authorMendoza Tesarik, Raquel
dc.contributor.authorGalán Lázaro, Maribel
dc.contributor.authorMendoza Ladrón De Guevara, Nicolás 
dc.contributor.authorTesarik, Jan
dc.contributor.authorAltmae, Signe 
dc.date.accessioned2022-02-22T07:53:50Z
dc.date.available2022-02-22T07:53:50Z
dc.date.issued2021-06-29
dc.identifier.citationNerea M. Molina... [et al.]. Assessing the testicular sperm microbiome: a low-biomass site with abundant contamination, Reproductive BioMedicine Online, Volume 43, Issue 3, 2021, Pages 523-531, ISSN 1472-6483, [https://doi.org/10.1016/j.rbmo.2021.06.021]es_ES
dc.identifier.urihttp://hdl.handle.net/10481/72935
dc.descriptionWe thank all men who generously donated testicular material for the purpose of this study. We also acknowledge the research support by Copan Italia S.p.A Inc., and Clearblue, SPD Swiss Precision Diagnostics GmbH. This study is part of a PhD Thesis conducted at the Official Doctoral Program in Biomedicine of the University of Granada, Spain. This work was supported by the Spanish Ministry of Economy, Industry and Competitiveness (MINECO) and European Regional Development Fund (FEDER): grant numbers RYC-2016-21199 and ENDORE (SAF2017-87526-R); by FEDER/Junta de Andalucia-Consejeria de Economia y Conocimiento: MENDO (B-CTS-500-UGR18); by Junta de Andalucia: (PAIDI P20_00158) by the University of Granada, Plan Propio de Investigacion 2016, Excellence actions: Units of Excellence; Unit of Excellence on Exercise and Health (UCEES), and the Junta de Andalucia, Consejeria de Conocimiento, Investigacion y Universidades and European Regional Development Fund: (SOMM17/6107/UGR); by Spanish Ministry of Science, Innovation, and Universities: (PRE2018085440 and FPU19/01638); and by Spanish Ministry of Education, Culture, and Sport: (FPU15/01193). Funding for open access charge: Universidad de Granada/CBUA Sequence data of all testicular spermatozoa and negative control samples have been deposited in the National Centre for Biotechnology Information (NCBI) Sequence Read Archive (SRA) database (http://www.ncbi.nlm.nih.gov/sra) under the BioProject ID PRJNA643898. The preliminary results of this study were presented as a poster communication at the 35th Annual ESHRE Meeting (Vienna, 2019).es_ES
dc.description.abstractResearch question: The semen harbours a diverse range of microorganisms. The origin of the seminal microbes, however, has not yet been established. Do testicular spermatozoa harbour microbes and could they potentially contribute to the seminal microbiome composition? Design: The study included 24 samples, comprising a total of 307 testicular maturing spermatozoa. A high-throughput sequencing method targeting V3 and V4 regions of 16S rRNA gene was applied. A series of negative controls together with stringent in-silico decontamination methods were analysed. Results: Between 50 and 70% of all the detected bacterial reads accounted for contamination in the testicular sperm samples. After stringent decontamination, Blautia (P = 0.04), Cellulosibacter (P = 0.02), Clostridium XIVa (P = 0.01), Clostridium XIVb (P = 0.04), Clostridium XVIII (P = 0.02), Collinsella (P = 0.005), Prevotella (P = 0.04), Prolixibacter (P = 0.02), Robinsoniella (P = 0.04), and Wandonia (P = 0.04) genera demonstrated statistically significant abundance among immature spermatozoa. Conclusions: Our results indicate that the human testicle harbours potential bacterial signature, though in a low-biomass, and could contribute to the seminal microbiome composition. Further, applying stringent decontamination methods is crucial for analysing microbiome in low-biomass site.es_ES
dc.description.sponsorshipCopan Italia S.p.A Inc.es_ES
dc.description.sponsorshipClearbluees_ES
dc.description.sponsorshipSPD Swiss Precision Diagnostics GmbHes_ES
dc.description.sponsorshipSpanish Governmentes_ES
dc.description.sponsorshipEuropean Commission RYC-2016-21199 SAF2017-87526-Res_ES
dc.description.sponsorshipFEDER/Junta de Andalucia-Consejeria de Economia y Conocimiento: MENDO B-CTS-500-UGR18 Junta de Andalucia PAIDI P20_00158es_ES
dc.description.sponsorshipUniversity of Granada, Plan Propio de Investigacion 2016, Excellence actions: Units of Excellencees_ES
dc.description.sponsorshipUnit of Excellence on Exercise and Health (UCEES)es_ES
dc.description.sponsorshipJunta de Andalucia Consejeria de Conocimiento, Investigacion y Universidadeses_ES
dc.description.sponsorshipEuropean Commission SOMM17/6107/UGRes_ES
dc.description.sponsorshipSpanish Government PRE2018085440 FPU19/01638 FPU15/01193es_ES
dc.description.sponsorshipUniversidad de Granada/CBUA Sequence BioProject PRJNA643898es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightsAtribución 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjectMale infertilityes_ES
dc.subjectMicrobiomees_ES
dc.subjectMicrobiotaes_ES
dc.subjectSemen es_ES
dc.subjectSpermes_ES
dc.titleAssessing the testicular sperm microbiome: a low-biomass site with abundant contaminationes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.doi10.1016/j.rbmo.2021.06.021
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones_ES


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