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New Red-Emitting Chloride-Sensitive Fluorescent Protein with Biological Uses

dc.contributor.authorSalto González, Rafael 
dc.contributor.authorGirón González, María Dolores 
dc.contributor.authorPuente Muñoz, Virginia
dc.contributor.authorVílchez Rienda, José Dámaso 
dc.contributor.authorEspinar Barranco, Laura
dc.contributor.authorValverde Pozo, Javier 
dc.contributor.authorParedes Martínez, José Manuel 
dc.date.accessioned2022-02-11T07:55:18Z
dc.date.available2022-02-11T07:55:18Z
dc.date.issued2021-06-21
dc.identifier.citationACS Sens. 2021, 6, 2563−2573. [https://doi.org/10.1021/acssensors.1c00094]es_ES
dc.identifier.urihttp://hdl.handle.net/10481/72792
dc.descriptionThis work was financially supported by CTQ2017-85685-R, CTQ2017-85454-C2-1-P, and CTQ2017-86125-P (MICIU/AEI/ERDF). J.M.P. and J.D.V. thank UEQ for funding. J.V.P. is supported by an FPU fellowship (FPU17/04749). D.A. was supported by the Italian Cystic Fibrosis Foundation (grant FFC#3/2019) and by Fondazione Cassa Rurale Trento Rovereto (ref 2018.256). The authors acknowledge the Universidad of Granada (Spain) cell culture and microscopy central facilities (CIC-UGR).es_ES
dc.description.abstractA new chloride-sensitive red fluorescent protein derived from Entacmaea quadricolor is described. We found that mBeRFP exhibited moderate sensitivity to chloride and, via sitedirected mutagenesis (S94V and R205Y), we increased the chloride affinity by more than an order of magnitude (kd = 106 ± 6 mM) at physiological pH. In addition, cis−trans isomerization of the chromophore produces a dual emission band with different chloride sensitivities, which allowed us to develop a ratiometric methodology to measure intracellular chloride concentrations.es_ES
dc.description.sponsorshipMICIU/AEI/ERDF CTQ2017-85685-R CTQ2017-85454-C2-1-P CTQ2017-86125-Pes_ES
dc.description.sponsorshipUEQes_ES
dc.description.sponsorshipSpanish Government FPU17/04749es_ES
dc.description.sponsorshipMinistry of Health, Italyes_ES
dc.description.sponsorshipItalian Cystic Fibrosis Research Foundation 3/2019es_ES
dc.description.sponsorshipFondazione Cassa Rurale Trento Rovereto 2018.256es_ES
dc.language.isoenges_ES
dc.publisherAmerican Chemical Societyes_ES
dc.rightsAtribución 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjectRed fluorescent proteinses_ES
dc.subjectChloride sensorses_ES
dc.subjectImaginges_ES
dc.subjectTwo-photon excitation microscopyes_ES
dc.subjectGenetically encoded sensorses_ES
dc.subjectIntracellular sensorses_ES
dc.titleNew Red-Emitting Chloride-Sensitive Fluorescent Protein with Biological Useses_ES
dc.typejournal articlees_ES
dc.rights.accessRightsopen accesses_ES
dc.identifier.doi10.1021/acssensors.1c00094
dc.type.hasVersionVoRes_ES


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