Mostrar el registro sencillo del ítem
New Red-Emitting Chloride-Sensitive Fluorescent Protein with Biological Uses
dc.contributor.author | Salto González, Rafael | |
dc.contributor.author | Girón González, María Dolores | |
dc.contributor.author | Puente Muñoz, Virginia | |
dc.contributor.author | Vílchez Rienda, José Dámaso | |
dc.contributor.author | Espinar Barranco, Laura | |
dc.contributor.author | Valverde Pozo, Javier | |
dc.contributor.author | Paredes Martínez, José Manuel | |
dc.date.accessioned | 2022-02-11T07:55:18Z | |
dc.date.available | 2022-02-11T07:55:18Z | |
dc.date.issued | 2021-06-21 | |
dc.identifier.citation | ACS Sens. 2021, 6, 2563−2573. [https://doi.org/10.1021/acssensors.1c00094] | es_ES |
dc.identifier.uri | http://hdl.handle.net/10481/72792 | |
dc.description | This work was financially supported by CTQ2017-85685-R, CTQ2017-85454-C2-1-P, and CTQ2017-86125-P (MICIU/AEI/ERDF). J.M.P. and J.D.V. thank UEQ for funding. J.V.P. is supported by an FPU fellowship (FPU17/04749). D.A. was supported by the Italian Cystic Fibrosis Foundation (grant FFC#3/2019) and by Fondazione Cassa Rurale Trento Rovereto (ref 2018.256). The authors acknowledge the Universidad of Granada (Spain) cell culture and microscopy central facilities (CIC-UGR). | es_ES |
dc.description.abstract | A new chloride-sensitive red fluorescent protein derived from Entacmaea quadricolor is described. We found that mBeRFP exhibited moderate sensitivity to chloride and, via sitedirected mutagenesis (S94V and R205Y), we increased the chloride affinity by more than an order of magnitude (kd = 106 ± 6 mM) at physiological pH. In addition, cis−trans isomerization of the chromophore produces a dual emission band with different chloride sensitivities, which allowed us to develop a ratiometric methodology to measure intracellular chloride concentrations. | es_ES |
dc.description.sponsorship | MICIU/AEI/ERDF CTQ2017-85685-R CTQ2017-85454-C2-1-P CTQ2017-86125-P | es_ES |
dc.description.sponsorship | UEQ | es_ES |
dc.description.sponsorship | Spanish Government FPU17/04749 | es_ES |
dc.description.sponsorship | Ministry of Health, Italy | es_ES |
dc.description.sponsorship | Italian Cystic Fibrosis Research Foundation 3/2019 | es_ES |
dc.description.sponsorship | Fondazione Cassa Rurale Trento Rovereto 2018.256 | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American Chemical Society | es_ES |
dc.rights | Atribución 3.0 España | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | Red fluorescent proteins | es_ES |
dc.subject | Chloride sensors | es_ES |
dc.subject | Imaging | es_ES |
dc.subject | Two-photon excitation microscopy | es_ES |
dc.subject | Genetically encoded sensors | es_ES |
dc.subject | Intracellular sensors | es_ES |
dc.title | New Red-Emitting Chloride-Sensitive Fluorescent Protein with Biological Uses | es_ES |
dc.type | journal article | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.identifier.doi | 10.1021/acssensors.1c00094 | |
dc.type.hasVersion | VoR | es_ES |