Identification of candidate genes involved in the determinism of pollen grain aperture morphology by comparative transcriptome analysis in Papaveraceae

Abstract

Supplementary Tables 3-6, 8-10: Table S3. Trinotate annotation report for Dactylicapnos torulosa. Annotation through blastx and blastp for predict transcript against Swissprot database. Table S4. Trinotate annotation report for Fumaria bracteosa. Annotation through blastx and blastp for predict transcript against Swissprot database. Table S5. Trinotate annotation report for Roemeria refracta. Annotation through blastx and blastp for predict transcript against Swissprot database. Table S6. Eschscholzia californica annotation through blastx against Swissprot database for transcripome transcript aligned to reference. Qseqid, query or source (e.g., gene) sequence id; seqid, subject or target (e.g., reference genome) sequence id; pident, percentage of identical matches; length, alignment length (sequence overlap); mismatch, number of mismatches; gapopen, number of gap openings; qstart, start of alignment in query; qend, end of alignment in query; sstart, start of alignment in subject; send, end of alignment in subject; evalue, expect value; bitscore, bit score. Table S8. Transcripts annotated as transcription factor through PlantTFDB. Table S9. Genes differentially expressed between colpate and porate species. Annotation through BLASTX searching against the SwissProt Database. Table S10. Functional classification of differentially expressed genes (DEGs) between colpate and porate species using Blast2GO software. Sheet 1, Blast2GO output file with annotations and functional classification for each DEG. Sheet2, summary of the number of DEGs for each functional annotation within the three different functional categories, note that each DEG can have several annotation possibilities.