Enhanced Cytotoxic Effect of TAT–PLGA-Embedded DOXO Carried by Biomimetic Magnetic Nanoparticles upon Combination with Magnetic Hyperthermia and Photothermia
Metadatos
Mostrar el registro completo del ítemAutor
Jabalera Ruz, Ylenia María; Sola Leyva, Alberto; Carrasco Jiménez, María Paz; Iglesias Salto, Guillermo Ramón; Jiménez López, ConcepciónEditorial
MDPI
Materia
Phothermia Hyperthermia BMNPs PLGA Magnetic nanoparticles TAT peptide Doxorubicin
Fecha
2021-07-28Referencia bibliográfica
Jabalera, Y... [et al.]. Enhanced Cytotoxic Effect of TAT–PLGA-Embedded DOXO Carried by Biomimetic Magnetic Nanoparticles upon Combination with Magnetic Hyperthermia and Photothermia. Pharmaceutics 2021, 13, 1168. [https://doi.org/10.3390/pharmaceutics13081168]
Patrocinador
Spanish Government CGL2016-76723; Instituto de Salud Carlos III Spanish Government; European Commission PID2019-109294RB-100; Spanish Government RYC-2014-16901; Junta de Andalucia A1-FQM-341-UGR18 C-FQM-497-UGR18 A-BIO-376-UGR18; Proyectos de I + D + I, del Plan Andaluz de Investigacion, Desarrollo e Innovacion P20_00208 P20_00346; Ministerio de Ciencia, Innovacion, y Universidades (Spain) PRE2018-085440; Ministerio de Educacion, Ciencia, y Deporte y Competitividad (Spain) FPU16_04580; Andalusian regional government CTS-236Resumen
The synergy between directed chemotherapy and thermal therapy (both magnetic hyperthermia
and photothermia) mediated by a nanoassembly composed of functionalized biomimetic
magnetic nanoparticles (BMNPs) with the chemotherapeutic drug doxorubicin (DOXO) covered by
the polymer poly(lactic-co-glycolic acid) (PLGA), decorated with TAT peptide (here referred to as
TAT–PLGA(DOXO-BMNPs)) is explored in the present study. The rationale behind this nanoassembly
lies in an optimization of the nanoformulation DOXO-BMNPs, already demonstrated to be
more efficient against tumor cells, both in vitro and in vivo, than systemic traditional therapies. By
embedding DOXO-BMNPs into PLGA, which is further functionalized with the cell-penetrating TAT
peptide, the resulting nanoassembly is able to mediate drug transport (using DOXO as a drug model)
and behaves as a hyperthermic agent (induced by an alternating magnetic field (AMF) or by laser
irradiation with a laser power density of 2 W/cm2). Our results obtained using the HepG2 cell line
show that there is a synergy between chemotherapy and thermal therapy that results in a stronger
cytotoxic effect when compared to that caused by the soluble DOXO. This is probably due to the
enhanced DOXO release occurring upon the application of the thermal therapy, as well as the induced
local temperature rise mediated by BMNPs in the nanoassembly following exposition to AMF or to
near-infrared (NIR) laser irradiation. These results represent a proof of concept demonstrating that
TAT–PLGA(DOXO-BMNPs) can be used to efficiently combine therapies against tumor cells, which
is a step forward in the transition from systemic to local treatments.