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dc.contributor.authorSánchez Cobos, Eva María 
dc.contributor.authorSánchez, Ignacio Enrique
dc.contributor.authorMurciano Calles, Javier 
dc.contributor.authorMartínez Herrerías, José Cristóbal
dc.identifier.citationCobos, E.S.; Sánchez, I.E.; Chemes, L.B.; Martinez, J.C.; Murciano-Calles, J. A Thermodynamic Analysis of the Binding Specificity between Four Human PDZ Domains and Eight Host, Viral and Designed Ligands. Biomolecules 2021, 11, 1071. 11081071es_ES
dc.description.abstractPDZ domains are binding modules mostly involved in cell signaling and cell–cell junctions. These domains are able to recognize a wide variety of natural targets and, among the PDZ partners, viruses have been discovered to interact with their host via a PDZ domain. With such an array of relevant and diverse interactions, PDZ binding specificity has been thoroughly studied and a traditional classification has grouped PDZ domains in three major specificity classes. In this work, we have selected four human PDZ domains covering the three canonical specificity-class binding mode and a set of their corresponding binders, including host/natural, viral and designed PDZ motifs. Through calorimetric techniques, we have covered the entire cross interactions between the selected PDZ domains and partners. The results indicate a rather basic specificity in each PDZ domain, with two of the domains that bind their cognate and some non-cognate ligands and the two other domains that basically bind their cognate partners. On the other hand, the host partners mostly bind their corresponding PDZ domain and, interestingly, the viral ligands are able to bind most of the studied PDZ domains, even those not previously described. Some viruses may have evolved to use of the ability of the PDZ fold to bind multiple targets, with resulting affinities for the virus–host interactions that are, in some cases, higher than for host–host interactions.es_ES
dc.description.sponsorshipAndalusian Regional Government, grant number CVI-5915es_ES
dc.description.sponsorshipANPCyT, grant numbers PICT 2012-2550 and PICT 2015-1213es_ES
dc.rightsAtribución 3.0 España*
dc.subjectPDZ domainses_ES
dc.subjectBinding specificityes_ES
dc.subjectVirus es_ES
dc.subjectIsothermal titration calorimetryes_ES
dc.subjectDifferential scanning calorimetryes_ES
dc.titleA Thermodynamic Analysis of the Binding Specificity between Four Human PDZ Domains and Eight Host, Viral and Designed Ligandses_ES
dc.identifier.doi10.3390/biom 11081071

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Atribución 3.0 España
Except where otherwise noted, this item's license is described as Atribución 3.0 España