dc.contributor.author | Sánchez, José Manuel | |
dc.contributor.author | Carretero, Irene | |
dc.contributor.author | González, Jorge | |
dc.contributor.author | Urrego, Diana | |
dc.contributor.author | Stühmer, Walter | |
dc.contributor.author | Pardo, Luis A. | |
dc.date.accessioned | 2021-06-23T08:03:25Z | |
dc.date.available | 2021-06-23T08:03:25Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | José Manuel Sánchez, Irene Carretero, Jorge González, Diana Urrego, Walter Stühmer and Luis A. Pardo. Primary cilium, cortactine, and Kv10.1 dynamics under confocal microscopy. AMU. 2016; 4: 27-30 | es_ES |
dc.identifier.issn | 2341-0361 | |
dc.identifier.uri | http://hdl.handle.net/10481/69345 | |
dc.description.abstract | OBJECTIVES: It is believed that tumor cells expressing
Kv10.1 channel acquire selective advantages that allow
them to maintain chronic proliferation. The ciliary
disassembly is a prerequisite to enter into the cell
cycle, therefore our objective was to find correlations
between primary cilium disassembly and the Kv10.1
channel through the Kv10.1 cortactin binding site.
Methods: Wild type Kv10.1, as well as the mutant form
lacking the binding site to the cytoskeleton protein
cortactin, were overexpressed in RPE-TERT cell line. The
effect on ciliary disassembly of such overexpression
forms was imaging using confocal microscopy.
Results: Kv10.1 wild type overexpression induced
disassembly of the primary cilium and alteration of the
cytoskeleton, which corresponded to overproduction
of filament structures positive to α-tubulin acetylated
in the cytoplasm. Overexpression of mutated Kv10.1
did not show this pattern, nor the untreated cells.
ConclusionS: This data suggests that Kv10.1
channel could be interfering with the cilium
formation, and thus influences the cell cycle in
a way that cortactine binding site is not involved.
That should be furtherly studied because due
to its possible implications in cancer. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Archivos de Medicina Universitaria | es_ES |
dc.rights | Atribución-NoComercial-SinDerivadas 3.0 España | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.subject | Kv10.1 channel | es_ES |
dc.subject | Primary cilium | es_ES |
dc.subject | Cortactin | es_ES |
dc.subject | Acetylated alpha-tubulin | es_ES |
dc.subject | Canal Kv10.1 | es_ES |
dc.subject | Cilio primario | es_ES |
dc.subject | Cortactina | es_ES |
dc.subject | Alfa-tubulina acetilada | es_ES |
dc.title | Primary cilium, cortactine, and Kv10.1 dynamics under confocal microscopy | es_ES |
dc.title.alternative | Estudio de lA dinámica del cilio primario y del canal de potasio Kv10.1 mediante microscopía confocal | es_ES |
dc.type | journal article | es_ES |
dc.rights.accessRights | open access | es_ES |