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dc.contributor.authorSánchez, José Manuel 
dc.contributor.authorCarretero, Irene
dc.contributor.authorGonzález, Jorge
dc.contributor.authorUrrego, Diana
dc.contributor.authorStühmer, Walter
dc.contributor.authorPardo, Luis A.
dc.date.accessioned2021-06-23T08:03:25Z
dc.date.available2021-06-23T08:03:25Z
dc.date.issued2016
dc.identifier.citationJosé Manuel Sánchez, Irene Carretero, Jorge González, Diana Urrego, Walter Stühmer and Luis A. Pardo. Primary cilium, cortactine, and Kv10.1 dynamics under confocal microscopy. AMU. 2016; 4: 27-30es_ES
dc.identifier.issn2341-0361
dc.identifier.urihttp://hdl.handle.net/10481/69345
dc.description.abstractOBJECTIVES: It is believed that tumor cells expressing Kv10.1 channel acquire selective advantages that allow them to maintain chronic proliferation. The ciliary disassembly is a prerequisite to enter into the cell cycle, therefore our objective was to find correlations between primary cilium disassembly and the Kv10.1 channel through the Kv10.1 cortactin binding site. Methods: Wild type Kv10.1, as well as the mutant form lacking the binding site to the cytoskeleton protein cortactin, were overexpressed in RPE-TERT cell line. The effect on ciliary disassembly of such overexpression forms was imaging using confocal microscopy. Results: Kv10.1 wild type overexpression induced disassembly of the primary cilium and alteration of the cytoskeleton, which corresponded to overproduction of filament structures positive to α-tubulin acetylated in the cytoplasm. Overexpression of mutated Kv10.1 did not show this pattern, nor the untreated cells. ConclusionS: This data suggests that Kv10.1 channel could be interfering with the cilium formation, and thus influences the cell cycle in a way that cortactine binding site is not involved. That should be furtherly studied because due to its possible implications in cancer.es_ES
dc.language.isoenges_ES
dc.publisherArchivos de Medicina Universitariaes_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectKv10.1 channeles_ES
dc.subjectPrimary ciliumes_ES
dc.subjectCortactines_ES
dc.subjectAcetylated alpha-tubulines_ES
dc.subjectCanal Kv10.1es_ES
dc.subjectCilio primarioes_ES
dc.subjectCortactinaes_ES
dc.subjectAlfa-tubulina acetiladaes_ES
dc.titlePrimary cilium, cortactine, and Kv10.1 dynamics under confocal microscopyes_ES
dc.title.alternativeEstudio de lA dinámica del cilio primario y del canal de potasio Kv10.1 mediante microscopía confocales_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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