Oncostatic effect of melatonin in head and neck cancer cells: clonogenic assay
Metadatos
Mostrar el registro completo del ítemAutor
González Díez, Manuel; Guerra Librero Rite, Ana; González Gil, Beatriz; Escames Rosa, GermaineEditorial
Archivos de Medicina Universitaria
Materia
Melatonin Irradiation HNSCC Oncostatic
Fecha
2015Referencia bibliográfica
González Díez, Manuel; Guerra-Librero, Ana; González Gil, Beatriz: Escames Rosa, Germaine. Oncostatic effect of melatonin in head and neck cancer cells: clonogenic assay. AMU. 2015; 3: 14-16
Resumen
Background: The oncostatic effect of melatonin
has been previously described among different
neoplastic types. One of these is head and neck
squamous cell cancer (HNSCC) with a high rate of
mortality and morbidity. Melatonin (aMT) could cause
cell death in this neoplastic cell type. To determine
this, we performed a clonogenic assay with CAL-27
cells treated with melatonin and/or radiation.
Methods: Cells were plated in a 6-well plate, with 800
cells per well. Assays were performed at least twice
and each time in triplicates. Cells were allowed to
grow 15 days to form colonies of at least 50 cells
each one. Cells were treated with melatonin (100,
500, 1000, 1500 and 2000 µM) alone or in combination
with irradiation (8 Gy). To visualize colonies, cells
were fixed in 100 % ethanol on days 12, 13, 14 and 15
after they were plated and stained with crystal violet
solution. Colonies were scored with Image J Software.
Results: The results clearly show that melatonin
inhibits colony growth of CAL-27 cells in a dosedependent manner in the groups treated with melatonin
alone 1500 µM or in combination with irradiation.
Conclusion: The results show the capability of aMT
to prevent colony growth and causing cell death on
CAL-27 cancer cells, especially when combined with
radiation. This is consistent with previous studies on
aMT oncostatic effects and suggests that usage of
aMT in vivo should have future clinical application.