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dc.contributor.authorGarzón Bello, Ingrid Johanna 
dc.contributor.authorJaimes Parra, Boris Damián
dc.contributor.authorCózar Olmo, José Manuel 
dc.contributor.authorSánchez Quevedo, María Del Carmen 
dc.contributor.authorSánchez-Montesinos García, Indalecio 
dc.contributor.authorFernández Valadés, Ricardo 
dc.contributor.authorCampos Sánchez, Fernando 
dc.contributor.authorAlaminos Mingorance, Miguel
dc.identifier.citationGarzón, I.; Jaimes-Parra, B.D.; Pascual-Geler, M.; Cózar, J.M.; Sánchez-Quevedo, M.d.C.; Mosquera-Pacheco, M.A.; Sánchez-Montesinos, I.; Fernández-Valadés, R.; Campos, F.; Alaminos, M. Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells. Polymers 2021, 13, 1568. 10.3390/polym13101568es_ES
dc.description.abstractSeveral models of bioartificial human urothelial mucosa (UM) have been described recently. In this study, we generated novel tubularized UM substitutes using alternative sources of cells. Nanostructured fibrin–agarose biomaterials containing fibroblasts isolated from the human ureter were used as stroma substitutes. Then, human Wharton jelly mesenchymal stromal cells (HWJSC) were used to generate an epithelial-like layer on top. Three differentiation media were used for 7 and 14 days. Results showed that the biofabrication methods used here succeeded in generating a tubular structure consisting of a stromal substitute with a stratified epithelial-like layer on top, especially using a medium containing epithelial growth and differentiation factors (EM), although differentiation was not complete. At the functional level, UM substitutes were able to synthesize collagen fibers, proteoglycans and glycosaminoglycans, although the levels of control UM were not reached ex vivo. Epithelial differentiation was partially achieved, especially with EM after 14 days of development, with expression of keratins 7, 8, and 13 and pancytokeratin, desmoplakin, tightjunction protein-1, and uroplakin 2, although at lower levels than controls. These results confirm the partial urothelial differentiative potential of HWJSC and suggest that the biofabrication methods explored here were able to generate a potential substitute of the human UM for future clinical use.es_ES
dc.description.sponsorshipCTS-115 Tissue Engineering Group and by the Spanish Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica, Ministry of Science and Innovation, Instituto de Salud Carlos III, grant FIS PI21/0981 (cofinanced by FEDER funds, European Union).es_ES
dc.rightsAtribución 3.0 España*
dc.subjectUrothelial mucosaes_ES
dc.subjectHuman Wharton jelly mesenchymal stromal cellses_ES
dc.titleBiofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cellses_ES

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Atribución 3.0 España
Except where otherwise noted, this item's license is described as Atribución 3.0 España