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dc.contributor.authorToledano Osorio, Manuel
dc.contributor.authorManzano Moreno, Francisco Javier
dc.contributor.authorToledano Pérez, Manuel 
dc.contributor.authorMedina Castillo, Antonio Luis 
dc.contributor.authorCostela Ruiz, Víctor Javier
dc.contributor.authorRuiz Rodríguez, Concepción 
dc.contributor.authorOsorio Ruiz, Raquel 
dc.date.accessioned2021-05-10T10:55:43Z
dc.date.available2021-05-10T10:55:43Z
dc.date.issued2021-03-28
dc.identifier.citationToledano-Osorio, M.; Manzano-Moreno, F.J.; Toledano, M.; Medina-Castillo, A.L.; Costela-Ruiz, V.J.; Ruiz, C.; Osorio, R. Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts. Polymers 2021, 13, 1063. [https://doi.org/10.3390/polym13071063]es_ES
dc.identifier.urihttp://hdl.handle.net/10481/68435
dc.descriptionThis research was funded by (1) the Ministry of Economy and Competitiveness and European Regional Development Fund [Project MAT2017-85999-P MINECO/AEI/FEDER/UE], (2) University of Granada/Regional Government of Andalusia Research Fund from Spain and European Regional Development Fund (A-BIO-157-UGR-18/FEDER). This research is part of Manuel Toledano-Osorio’s PhD research study.es_ES
dc.description.abstractPolymeric membranes are employed in guided bone regeneration (GBR) as physical barriers to facilitate bone in-growth. A bioactive and biomimetic membrane with the ability to participate in the healing and regeneration of the bone is necessary. The aim of the present study was to analyze how novel silicon dioxide composite membranes functionalized with zinc or doxycycline can modulate the osteoblasts' proliferation, differentiation, and expression of selected antigenic markers related to immunomodulation. Nanostructured acrylate-based membranes were developed, blended with silica, and functionalized with zinc or doxycycline. They were subjected to MG63 osteoblast-like cells culturing. Proliferation was assessed by MTT-assay, differentiation by evaluating the alkaline phosphatase activity by a spectrophotometric method and antigenic phenotype was assessed by flow cytometry for selected markers. Mean comparisons were conducted by one-way ANOVA and Tukey tests (p < 0.05). The blending of silica nanoparticles in the tested non-resorbable polymeric scaffold improved the proliferation and differentiation of osteoblasts, but doxycycline doped scaffolds attained the best results. Osteoblasts cultured on doxycycline functionalized membranes presented higher expression of CD54, CD80, CD86, and HLA-DR, indicating a beneficial immunomodulation activity. Doxycycline doped membranes may be a potential candidate for use in GBR procedures in several challenging pathologies, including periodontal disease.es_ES
dc.description.sponsorshipEuropean Commission MAT2017-85999-P A-BIO-157-UGR-18/FEDERes_ES
dc.description.sponsorshipMinistry of Economy and Competitiveness MAT2017-85999-Pes_ES
dc.description.sponsorshipUniversity of Granada/Regional Government of Andalusia Research Fund from Spain A-BIO-157-UGR-18/FEDERes_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightsAtribución 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjectCD markerses_ES
dc.subjectDoxycyclinees_ES
dc.subjectAntigenic phenotypees_ES
dc.subjectOsteoblastses_ES
dc.subjectMembranees_ES
dc.titleDoxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblastses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.doi10.3390/polym13071063
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones_ES


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Atribución 3.0 España
Except where otherwise noted, this item's license is described as Atribución 3.0 España