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dc.contributor.authorSilva, Duílio M. Z. de A.
dc.contributor.authorRuiz-Ruano, Francisco J.
dc.contributor.authorMartín Peciña, María
dc.contributor.authorMartínez Camacho, Juan Pedro
dc.identifier.citationSilva, D.M., Ruiz-Ruano, F.J., Utsunomia, R. et al. Long-term persistence of supernumerary B chromosomes in multiple species of Astyanax fish. BMC Biol 19, 52 (2021). []es_ES
dc.descriptionWe are thankful Renato Devidé for his technical assistance and Rafael Henrique Nóbrega and Maeli Dal Pai Silva for the use of their laboratory equipment and Judith Mank for the useful feedback to the manuscript.es_ES
dc.description.abstractBackground: Eukaryote genomes frequently harbor supernumerary B chromosomes in addition to the “standard” A chromosome set. B chromosomes are thought to arise as byproducts of genome rearrangements and have mostly been considered intraspecific oddities. However, their evolutionary transcendence beyond species level has remained untested. Results: Here we reveal that the large metacentric B chromosomes reported in several fish species of the genus Astyanax arose in a common ancestor at least 4 million years ago. We generated transcriptomes of A. scabripinnis and A. paranae 0B and 1B individuals and used these assemblies as a reference for mapping all gDNA and RNA libraries to quantify coverage differences between B-lacking and B-carrying genomes. We show that the B chromosomes of A. scabripinnis and A. paranae share 19 protein-coding genes, of which 14 and 11 were also present in the B chromosomes of A. bockmanni and A. fasciatus, respectively. Our search for B-specific singlenucleotide polymorphisms (SNPs) identified the presence of B-derived transcripts in B-carrying ovaries, 80% of which belonged to nobox, a gene involved in oogenesis regulation. Importantly, the B chromosome nobox paralog is expressed > 30× more than the A chromosome paralog. This indicates that the normal regulation of this gene is altered in B-carrying females, which could potentially facilitate B inheritance at higher rates than Mendelian law prediction. Conclusions: Taken together, our results demonstrate the long-term survival of B chromosomes despite their lack of regular pairing and segregation during meiosis and that they can endure episodes of population divergence leading to species formation.es_ES
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) 2014/09634-5 2013/24143-5 2017/22447-8 2018/20610-1 2016/09204-6 2014/26508-3es_ES
dc.description.sponsorshipNational Council for Scientific and Technological Development (CNPq) 306054/2006-0es_ES
dc.description.sponsorshipSpanish Secretaria de Estado de Investigacion, Desarrollo e Innovacion CGL2015-70750-Pes_ES
dc.description.sponsorshipJunta de Andaluciaes_ES
dc.description.sponsorshipSven och Lilly Lawskis fond (Sweden)es_ES
dc.description.sponsorshipMarie Sklodowska-Curie Individual Fellowship (European Commission) 875732es_ES
dc.description.sponsorshipUppsala Universityes_ES
dc.rightsAtribución 3.0 España*
dc.subjectB chromosomeses_ES
dc.subjectEvolution es_ES
dc.titleLong-term persistence of supernumerary B chromosomes in multiple species of Astyanax fishes_ES

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Atribución 3.0 España
Except where otherwise noted, this item's license is described as Atribución 3.0 España