Mostrar el registro sencillo del ítem
Protein O-Fucosyltransferase 1 Undergoes Interdomain Flexibility in Solution
dc.contributor.author | Lira Navarrete, Erandi | |
dc.contributor.author | Castello, Fabio | |
dc.contributor.author | Ruedas Rama, María José | |
dc.contributor.author | Orte Gutiérrez, Ángel | |
dc.date.accessioned | 2021-04-13T10:04:03Z | |
dc.date.available | 2021-04-13T10:04:03Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Lira-Navarrete, E.; Pallarés, M.C.; Castello, F.; Ruedas-Rama, M.J.; Orte, A.; Lostao, A.; Hurtado-Guerrero, R. Protein O-Fucosyltransferase 1 Undergoes Interdomain Flexibility in Solution. Molecules 2021, 26, 2105. https:// doi.org/10.3390/molecules26082105 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10481/67929 | |
dc.description.abstract | Protein O-fucosyltransferase 1 (PoFUT1) is a GT-B fold enzyme that fucosylates proteins containing EGF-like repeats. GT-B glycosyltransferases have shown a remarkable grade of plasticity adopting closed and open conformations as a way of tuning their catalytic cycle, a feature that has not been observed for PoFUT1. Here, we analyzed Caenorhabditis elegans PoFUT1 (CePoFUT1) conformational behavior in solution by atomic force microscopy (AFM) and single-molecule fluorescence resonance energy transfer (SMF-FRET). Our results show that this enzyme is very flexible and adopts mainly compact conformations and to a lesser extend a highly dynamic population that oscillates between compact and highly extended conformations. Overall, our experiments illustrate the inherent complexity of CePoFUT1 dynamics, which might play a role during its catalytic cycle. | es_ES |
dc.description.sponsorship | ARAID: MEC (CTQ2013-44367-C2-2-P, BFU2016-75633-P and PID2019-105451GBI00 to RH-G, CTQ2017-85658-R and CTQ2014-56370-R to AO) | es_ES |
dc.description.sponsorship | Gobierno de Aragón (E35_R20 and LMP58_18) | es_ES |
dc.description.sponsorship | FEDER (2014-2020) funds for ‘Building Europe from Aragón’ | es_ES |
dc.description.sponsorship | Juan de la Cierva fellowship IJCI-2017-32874 | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | Atribución 3.0 España | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | Glycosyltransferases | es_ES |
dc.subject | O-fucosylation | es_ES |
dc.subject | Protein dynamics | es_ES |
dc.subject | Atomic force microscopy | es_ES |
dc.subject | Singlemolecule methods | es_ES |
dc.title | Protein O-Fucosyltransferase 1 Undergoes Interdomain Flexibility in Solution | es_ES |
dc.type | journal article | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.identifier.doi | 10.3390/molecules26082105 |