Anemonia sulcata and Its Symbiont Symbiodinium as a Source of Anti-Tumor and Anti-Oxoxidant Compounds for Colon Cancer Therapy: A Preliminary in Vitro Study
Metadatos
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Cabeza Montilla, Laura; Peña Contreras, María Mercedes; Martínez Martínez, Rosario; Mesas Hernández, Cristina; Galisteo Moya, Milagros; Perazzoli, Gloria; Prados Salazar, José Carlos; Porres Foulquie, Jesús María; Melguizo Alonso, ConsolaciónEditorial
Mdpi
Materia
Anemonia sulcata Symbiodinium Antioxidant activity Antitumor activity Colorectal cancer
Fecha
2021-02-08Referencia bibliográfica
Cabeza, L.; Peña, M.; Martínez, R.; Mesas, C.; Galisteo, M.; Perazzoli, G.; Prados, J.; Porres, J.M.; Melguizo, C. Anemonia sulcata and Its Symbiont Symbiodinium as a Source of Anti-Tumor and Anti-Oxoxidant Compounds for Colon Cancer Therapy: A Preliminary In Vitro Study. Biology 2021, 10, 134. [https://doi.org/10.3390/biology10020134]
Patrocinador
International Excellence Campus of the Sea (CEI.MAR) CEIJ-007 CEIJ-0012Resumen
Simple Summary: Colorectal cancer is one of the most frequent types of cancer in the population.
Recently, invertebrate marine animals have been investigated for the presence of natural products
which can damage tumor cells, prevent their spread to other tissues or avoid cancer develop. We
analyzed the anemone Anemonia sulcata with and without the presence of its microalgal symbiont
(Symbiodinium) as a source of bioactive molecules for the colorectal cancer therapy and prevention.
Colon cancer tumor cells were exposed to Anemone extracts observing a remarkable cell death and
a great antioxidant capacity. These preliminary results support that Anemonia sulcata could be a
source of bioactive compounds against colorectal cancer and that the absence of its symbiont may
enhance these properties. Further studies will be necessary to define the bioactive compounds of
Anemonia sulcata and their mechanisms of action.
Abstract: Recently, invertebrate marine species have been investigated for the presence of natural
products with antitumor activity. We analyzed the invertebrate Anemonia sulcata with (W) and without
(W/O) the presence of its microalgal symbiont Symbiodinium as a source of bioactive compounds
that may be applied in the therapy and/or prevention of colorectal cancer (CRC). Animals were
mechanically homogenized and subjected to ethanolic extraction. The proximate composition and
fatty acid profile were determined. In addition, an in vitro digestion was performed to study the
potentially dialyzable fraction. The antioxidant and antitumor activity of the samples and the digestion
products were analyzed in CRC cells in vitro. Our results show a high concentration of
polyunsaturated fatty acid in the anemone and a great antioxidant capacity, which demonstrated
the ability to prevent cell death and a high antitumor activity of the crude homogenates against
CRC cells and multicellular tumor spheroids, especially W/O symbiont. These preliminary results
support that Anemonia sulcata could be a source of bioactive compounds with antioxidant and antitumor
potential against CRC and that the absence of its symbiont may enhance these properties.
Further studies will be necessary to define the bioactive compounds of Anemonia sulcata and their
mechanisms of action.