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dc.contributor.authorMarcos Torres, Francisco Javier
dc.contributor.authorPérez Torres, Juana 
dc.contributor.authorGómez-Santos, Nuria
dc.contributor.authorMoraleda Muñoz, Aurelio 
dc.contributor.authorMuñoz Dorado, José 
dc.date.accessioned2021-03-19T12:26:22Z
dc.date.available2021-03-19T12:26:22Z
dc.date.issued2016-07-08
dc.identifier.citationMarcos-Torres FJ, Pérez J, Gómez-Santos N, Moraleda-Muñoz A, Muñoz-Dorado J. In depth analysis of the mechanism of action of metal-dependent sigma factors: characterization of CorE2 from Myxococcus xanthus. Nucleic Acids Res. 2016 Jul 8;44(12):5571-84. doi: 10.1093/nar/gkw150. Epub 2016 Mar 6. PMID: 26951374; PMCID: PMC4937300.es_ES
dc.identifier.urihttp://hdl.handle.net/10481/67356
dc.description.abstractExtracytoplasmic function sigma factors represent the third pillar of signal-transduction mechanisms in bacteria. The variety of stimuli they recognize and mechanisms of action they use have allowed their classification into more than 50 groups. We have characterized CorE2 from Myxococcus xanthus, which belongs to group ECF44 and upregulates the expression of two genes when it is activated by cadmium and zinc. Sigma factors of this group contain a Cys-rich domain (CRD) at the C terminus which is essential for detecting metals. Point mutations at the six Cys residues of the CRD have revealed the contribution of each residue to CorE2 activity. Some of them are essential, while others are either dispensable or their mutations only slightly affect the activity of the protein. However, importantly, mutation of Cys174 completely shifts the specificity of CorE2 from cadmium to copper, indicating that the Cys arrangement of the CRD determines the metal specificity. Moreover, the conserved CxC motif located between the sigma2 domain and the sigma4.2 region has also been found to be essential for activity. The results presented here contribute to our understanding of the mechanism of action of metal-dependent sigma factors and help to define new common features of the members of this group of regulators.es_ES
dc.description.sponsorshipSpanish Government [CSD2009-00006, BFU2012-33248, 70% funded by FEDER]. Funding for open access charge: Grupo BIO318, Junta de Andalucía.es_ES
dc.language.isoenges_ES
dc.publisherOxford University Presses_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectTranscriptiones_ES
dc.subjectECF sigma factores_ES
dc.subjectBacterial adaptationes_ES
dc.subjectSignal transductiones_ES
dc.subjectMetals es_ES
dc.titleIn depth analysis of the mechanism of action of metal-dependent sigma factors: characterization of CorE2 from Myxococcus xanthuses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.doi10.1093/nar/gkw150
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones_ES


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