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dc.contributor.authorGarcía García, Óscar Darío
dc.contributor.authorEl Soury, Marwa
dc.contributor.authorGonzález Quevedo, David
dc.contributor.authorSánchez Porras, David 
dc.contributor.authorChato Astrain, Jesús 
dc.contributor.authorCampos, Fernando
dc.contributor.authorCarriel Araya, Víctor 
dc.date.accessioned2021-03-04T08:58:02Z
dc.date.available2021-03-04T08:58:02Z
dc.date.issued2021
dc.identifier.citationGarcía-García, Ó.D.; El Soury, M.; González-Quevedo, D.; Sánchez-Porras, D.; Chato-Astrain, J.; Campos, F.; Carriel, V. Histological, Biomechanical, and Biological Properties of Genipin-Crosslinked Decellularized Peripheral Nerves. Int. J. Mol. Sci. 2021, 22, 674. https:// doi.org/10.3390/ijms22020674es_ES
dc.identifier.urihttp://hdl.handle.net/10481/66862
dc.description.abstractAcellular nerve allografts (ANGs) represent a promising alternative in nerve repair. Our aim is to improve the structural and biomechanical properties of biocompatible Sondell (SD) and Roosens (RS) based ANGs using genipin (GP) as a crosslinker agent ex vivo. The impact of two concentrations of GP (0.10% and 0.25%) on Wistar rat sciatic nerve-derived ANGs was assessed at the histological, biomechanical, and biocompatibility levels. Histology confirmed the differences between SD and RS procedures, but not remarkable changes were induced by GP, which helped to preserve the nerve histological pattern. Tensile test revealed that GP enhanced the biomechanical properties of SD and RS ANGs, being the crosslinked RS ANGs more comparable to the native nerves used as control. The evaluation of the ANGs biocompatibility conducted with adipose-derived mesenchymal stem cells cultured within the ANGs confirmed a high degree of biocompatibility in all ANGs, especially in RS and RS-GP 0.10% ANGs. Finally, this study demonstrates that the use of GP could be an efficient alternative to improve the biomechanical properties of ANGs with a slight impact on the biocompatibility and histological pattern. For these reasons, we hypothesize that our novel crosslinked ANGs could be a suitable alternative for future in vivo preclinical studies.es_ES
dc.description.sponsorshipSpanish "Plan Nacional de Investigacion Cientifica, Desarrollo e Innovacion Tecnologica, Ministerio de Economia y Competitividad (Instituto de Salud Carlos III) FIS PI14-1343 FIS PI17-0393 FIS PI20-0318es_ES
dc.description.sponsorshipFondo Europeo de Desarrollo Regional ERDF-FEDER European Uniones_ES
dc.description.sponsorshipPlan Andaluz de Investigacion, Desarrollo e Innovacion (PAIDI 2020), Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades, Junta de Andalucia, Espana P18-RT-5059es_ES
dc.description.sponsorshipPrograma Operativo FEDER Andalucia 2014-2020, Universidad de Granada, Junta de Andalucia, Espana A-CTS-498UGR18es_ES
dc.description.sponsorshipERDF-FEDER, the European Uniones_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightsAtribución 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjectTissue engineeringes_ES
dc.subjectNerve repaires_ES
dc.subjectNerve tissue decellularizationes_ES
dc.subjectGenipines_ES
dc.subjectChemical crosslinkinges_ES
dc.subjectHistology es_ES
dc.subjectNatural biomaterialses_ES
dc.subjectBiomechanical and structural propertieses_ES
dc.subjectCellbiomaterials interactionses_ES
dc.titleHistological, Biomechanical, and Biological Properties of Genipin-Crosslinked Decellularized Peripheral Nerveses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.doi10.3390/ijms22020674


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Atribución 3.0 España
Except where otherwise noted, this item's license is described as Atribución 3.0 España