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dc.contributor.authorPlaza Díaz, Julio 
dc.contributor.authorRobles-Sánchez, Candido
dc.contributor.authorAbadía Molina, Francisco 
dc.contributor.authorSáez Lara, María José 
dc.contributor.authorVílchez-Padial, Laura María
dc.contributor.authorGil Hernández, Ángel 
dc.contributor.authorGómez Llorente, Carolina 
dc.contributor.authorFontana Gallego, Luis 
dc.date.accessioned2021-02-08T13:24:29Z
dc.date.available2021-02-08T13:24:29Z
dc.date.issued2017-12-12
dc.identifier.citationPlaza-Díaz, J., Robles-Sánchez, C., Abadía-Molina, F. et al. Gene expression profiling in the intestinal mucosa of obese rats administered probiotic bacteria. Sci Data 4, 170186 (2017). [https://doi.org/10.1038/sdata.2017.186]es_ES
dc.identifier.urihttp://hdl.handle.net/10481/66392
dc.descriptionThis paper will be part of Candido Robles-Sanchez's doctorate, which is being completed as part of the 'Biochemistry and Molecular Biology Program' at the University of Granada, Spain. This work was funded by the company Hero Spain, S. A. (grant #3545 managed by Fundacion General EmpresaUniversidad de Granada) and by CEIBiotic, University of Granada, Spain (grant CEI2013P-11).es_ES
dc.description.abstractWe investigated whether the administration of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 modulate the expression of genes in the intestinal mucosa of obese Zucker rats. Forty-eight Zucker-Lepr(fa/fa) and 16 Zucker lean Lepr(+/fa) rats were used. Eight Zucker lean Lepr(+/fa) and 8 Zucker-Lepr(fa/fa) rats were euthanized as a reference. The remaining 40 Zucker-Lepr(fa/fa) rats were then assigned to receive 10(10) colony forming units (CFU) of one of the three probiotic strains, a mixture of L. paracasei CNCM I-4034 and B. breve CNCM I-4035, or a placebo by oral administration for 30 days. An additional group of 8 Zucker lean Lepr(+/fa) rats received the placebo for 30 days. Over 27,000 rat genes were studied using a DNA array. Four animals per group were used. Total RNA was extracted from intestinal mucosa and cDNA was synthesized, fragmented and labeled. Labeled cDNA was hybridized using GeneChip kits, and the latter were scanned. Intensity values of each probe were processed and normalized to obtain an individual value for each set of probes.es_ES
dc.description.sponsorshipCompany Hero Spain, S. A. 3545es_ES
dc.description.sponsorshipCEIBiotic, University of Granada, Spain CEI2013P-11es_ES
dc.language.isoenges_ES
dc.publisherSpringer Naturees_ES
dc.rightsAtribución 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjectApplied microbiologyes_ES
dc.subjectMetabolic disorderses_ES
dc.titleGene expression profiling in the intestinal mucosa of obese rats administered probiotic bacteriaes_ES
dc.typedatasetes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.doi10.1038/sdata.2017.186
dc.identifier.doi10.30827/Digibug.66392
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones_ES
dc.publication.year2017


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Atribución 3.0 España
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