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dc.contributor.authorPeinado, Paola
dc.contributor.authorAndrades Delgado, Álvaro 
dc.contributor.authorCuadros Celorrio, Marta Eugenia 
dc.contributor.authorRodríguez Lara, María Isabel
dc.contributor.authorGarcía García, Daniel Jesús
dc.contributor.authorÁlvarez Pérez, Juan Carlos
dc.contributor.authorBaliñas Gavira, Carlos
dc.contributor.authorArenas Molina, Alberto Manuel
dc.contributor.authorPatiño Mercau, Juan Rodrigo
dc.contributor.authorSanjuan Hidalgo, Juan
dc.contributor.authorMedina Vico, Pedro Pablo
dc.identifier.citationPeinado, P., Andrades, A., Cuadros, M., Rodriguez, M. I., Coira, I. F., Garcia, D. J., ... & Medina, P. P. (2020). Comprehensive Analysis of SWI/SNF Inactivation in Lung Adenocarcinoma Cell Models. Cancers, 12(12), 3712. [doi:10.3390/cancers12123712]es_ES
dc.description.abstractSimple Summary: Mammalian SWI/SNF complexes regulate gene expression by reorganizing the way DNA is packaged into chromatin. SWI/SNF subunits are recurrently altered in tumors at multiple levels, including DNA mutations as well as alteration of the levels of RNA and protein. Cancer cell lines are often used to study SWI/SNF function, but their patterns of SWI/SNF alterations can be complex. Here, we present a comprehensive characterization of DNA mutations and RNA and protein expression of SWI/SNF members in 38 lung adenocarcinoma (LUAD) cell lines. We show that over 85% of our cell lines harbored at least one alteration in one SWI/SNF subunit. In addition, over 75% of our cell lines lacked expression of at least one SWI/SNF subunit at the protein level. Our catalog will help researchers choose an appropriate cell line model to study SWI/SNF function in LUAD. Abstract: Mammalian SWI/SNF (SWitch/Sucrose Non-Fermentable) complexes are ATP-dependent chromatin remodelers whose subunits have emerged among the most frequently mutated genes in cancer. Studying SWI/SNF function in cancer cell line models has unveiled vulnerabilities in SWI/SNF-mutant tumors that can lead to the discovery of new therapeutic drugs. However, choosing an appropriate cancer cell line model for SWI/SNF functional studies can be challenging because SWI/SNF subunits are frequently altered in cancer by various mechanisms, including genetic alterations and post-transcriptional mechanisms. In this work, we combined genomic, transcriptomic, and proteomic approaches to study the mutational status and the expression levels of the SWI/SNF subunits in a panel of 38 lung adenocarcinoma (LUAD) cell lines. We found that the SWI/SNF complex was mutated in more than 76% of our LUAD cell lines and there was a high variability in the expression of the di erent SWI/SNF subunits. These results underline the importance of the SWI/SNF complex as a tumor suppressor in LUAD and the di culties in defining altered and unaltered cell models for the SWI/SNF complex. These findings will assist researchers in choosing the most suitable cellular models for their studies of SWI/SNF to bring all of its potential to the development of novel therapeutic applications.es_ES
dc.description.sponsorshipMinistry of Economy of Spain SAF2015-67919-Res_ES
dc.description.sponsorshipJunta de Andalucía CS2016-3 P12-BIO1655 PIGE-0440-2019 Pl-0245-2017 PI-0135-2020es_ES
dc.description.sponsorshipUniversity of Granada PPJIA2019-0 B-CTS-126-UGR18es_ES
dc.description.sponsorshipInternational Association for the Study of Lung Cancer (IASLC)es_ES
dc.description.sponsorshipSpanish Association for Cancer Research (LAB-AECC)es_ES
dc.description.sponsorshipPhD "La Caixa Foundation" LCF/BQ/DE15/10360019es_ES
dc.description.sponsorship"Fundacion Benefica Anticancer Santa Candida y San Francisco Javier" predoctoral fellowshipes_ES
dc.description.sponsorshipEuropean Commission 837897es_ES
dc.description.sponsorshipSpanish Ministry of Education, Culture and Sports FPU fellowship FPU17/00067 FPU17/01258 FPU18/03709es_ES
dc.description.sponsorshipPhD FPI-fellowship BES-2013-064596es_ES
dc.description.sponsorshipFundación Científica de la Asociación Española Contra el Cáncer GCB14-2170es_ES
dc.description.sponsorshipFundación Ramon Areceses_ES
dc.description.sponsorshipInstituto de Salud Carlos III-Fondo de Investigación Sanitaria-Fondo Europeo de Desarrollo Regional `Una manera de hacer Europa' (FEDER) PI19/00098es_ES
dc.rightsAtribución 3.0 España*
dc.subjectSWI/SNF complexes_ES
dc.subjectLung canceres_ES
dc.subjectLung adenocarcinomaes_ES
dc.subjectCell modeles_ES
dc.titleComprehensive Analysis of SWI/SNF Inactivation in Lung Adenocarcinoma Cell Modelses_ES

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Atribución 3.0 España
Except where otherwise noted, this item's license is described as Atribución 3.0 España