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dc.contributor.authorPalacios Ferrer, José Luis
dc.contributor.authorGarcía Ortega, María Belén
dc.contributor.authorGarcía Chaves, María Ángel 
dc.contributor.authorDíaz, Caridad
dc.contributor.authorBoulaiz Tassi, Houria 
dc.contributor.authorValdivia, Javier
dc.contributor.authorJurado, José Miguel
dc.contributor.authorAlmazán Fernández, Francisco Manuel
dc.contributor.authorArias Santiago, Salvador
dc.contributor.authorAmezcua, Víctor
dc.contributor.authorPeinado, Héctor
dc.contributor.authorVicente, Francisca
dc.contributor.authorPérez del Palacio, José
dc.contributor.authorMarchal Corrales, Juan Antonio
dc.identifier.citationPalacios‐Ferrer, J. L., García‐Ortega, M. B., Gallardo‐Gómez, M., García, M. Á., Díaz, C., Boulaiz, H., ... & Marchal, J. A. (2020). Metabolomic profile of cancer stem cell‐derived exosomes from patients with malignant melanoma. Molecular Oncology. [doi:10.1002/1878-0261.12823]es_ES
dc.description.abstractMalignant melanoma (MM) is the most aggressive and life-threatening form of skin cancer. It is characterized by an extraordinary metastasis capacity and chemotherapy resistance, mainly due to melanoma cancer stem cells (CSCs). To date, there are no suitable clinical diagnostic, prognostic or predictive biomarkers for this neoplasia. Therefore, there is an urgent need for new MM biomarkers that enable early diagnosis and effective disease monitoring. Exosomes represent a novel source of biomarkers since they can be easily isolated from different body fluids. In this work, a primary patient-derived MM cell line enriched in CSCs was characterized by assessing the expression of specific markers and their stem-like properties. Exosomes derived from CSCs and serums from patients with MM were characterized, and their metabolomic profile was analysed by highresolution mass spectrometry (HRMS) following an untargeted approach and applying univariate and multivariate statistical analyses. The aim of this study was to search potential biomarkers for the diagnosis of this disease. Our results showed significant metabolomic differences in exosomes derived from MM CSCs compared with those from differentiated tumour cells and also in serum-derived exosomes from patients with MM compared to those from healthy controls. Interestingly, we identified similarities between structural lipids differentially expressed in CSC-derived exosomes and those derived from patients with MM such as the glycerophosphocholine PC 16:0/0:0. To our knowledge, this is the first metabolomic-based study aimed at characterizing exosomes derived from melanoma CSCs and patients’ serum in order to identify potential biomarkers for MM diagnosis. We conclude that metabolomic characterization of CSC-derived exosomes sets an open door to the discovery of clinically useful biomarkers in this neoplasia.es_ES
dc.description.sponsorshipMICIU FPU15/03682 FPU15/02350es_ES
dc.description.sponsorshipMinisterio de Ciencia, Innovación y Universidades (MICIU) MAT2015-62644.C2.2.R RTI2018-101309-BC2es_ES
dc.description.sponsorshipInstituto de Salud Carlos III PIE16-00045es_ES
dc.description.sponsorshipJunta de Andalucía SOMM17/6109/UGR (UCE-PP2017-3)es_ES
dc.description.sponsorshipEuropean Union (EU) SOMM17/6109/UGR (UCE-PP2017-3)es_ES
dc.description.sponsorshipChair 'Doctors Galera-Requena in cancer stem cell research' CMC-CTS963es_ES
dc.description.sponsorshipFundación MEDINAes_ES
dc.rightsAtribución 3.0 España*
dc.subjectCancer stem cellses_ES
dc.subjectMalignant melanomaes_ES
dc.titleMetabolomic profile of cancer stem cell-derived exosomes from patients with malignant melanomaes_ES

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Atribución 3.0 España
Except where otherwise noted, this item's license is described as Atribución 3.0 España