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dc.contributor.authorMartínez Molina, Eduardo
dc.contributor.authorChocarro Wrona, Carlos
dc.contributor.authorMartínez Moreno, Daniel
dc.contributor.authorMarchal Corrales, Juan Antonio 
dc.contributor.authorBoulaiz Tassi, Houria
dc.identifier.citationMartínez-Molina, E., Chocarro-Wrona, C., Martínez-Moreno, D., Marchal, J. A., & Boulaiz, H. (2020). Large-Scale Production of Lentiviral Vectors: Current Perspectives and Challenges. Pharmaceutics, 12(11), 1051. [doi:10.3390/pharmaceutics12111051]es_ES
dc.description.abstractLentiviral vectors (LVs) have gained value over recent years as gene carriers in gene therapy. These viral vectors are safer than what was previously being used for gene transfer and are capable of infecting both dividing and nondividing cells with a long-term expression. This characteristic makes LVs ideal for clinical research, as has been demonstrated with the approval of lentivirus-based gene therapies from the Food and Drug Administration and the European Agency for Medicine. A large number of functional lentiviral particles are required for clinical trials, and large-scale production has been challenging. Therefore, e orts are focused on solving the drawbacks associated with the production and purification of LVsunder current good manufacturing practice. In recent years, we have witnessed the development and optimization of new protocols, packaging cell lines, and culture devices that are very close to reaching the target production level. Here, we review the most recent, e cient, and promising methods for the clinical-scale production ofLVs.es_ES
dc.description.sponsorshipFundación Empresa Universidad de Granada PR/18/001es_ES
dc.description.sponsorshipInstituto de Salud Carlos III FMM-AP16683-2017es_ES
dc.description.sponsorshipJunta de Andalucía PI-0089-2017es_ES
dc.description.sponsorshipChair "Doctors Galera-Requena in cancer stem cell research"es_ES
dc.description.sponsorshipConsejería de Economía, Conocimiento, Empresas y Universidades de la Junta de Andalucía (FEDER Funds) SOMM17/6109/UGRes_ES
dc.rightsAtribución 3.0 España*
dc.subjectLentiviral vectores_ES
dc.subjectGene therapy es_ES
dc.subjectLarge-scale productiones_ES
dc.titleLarge-Scale Production of Lentiviral Vectors: Current Perspectives and Challengeses_ES

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Atribución 3.0 España
Except where otherwise noted, this item's license is described as Atribución 3.0 España