Lipid Accumulation in HepG2 Cells Is Attenuated by Strawberry Extract through AMPK Activation
Metadatos
Mostrar el registro completo del ítemEditorial
MDPI
Materia
Strawberry Cholesterol synthesis Fatty acids synthesis Hypolipidemic agent
Fecha
2017Referencia bibliográfica
Forbes-Hernández, T.Y.; Giampieri, F.; Gasparrini, M.; Afrin, S.; Mazzoni, L.; Cordero, M.D.; Mezzetti, B.; Quiles, J.L.; Battino, M. Lipid Accumulation in HepG2 Cells Is Attenuated by Strawberry Extract through AMPK Activation. Nutrients 2017, 9, 621. [https://doi.org/10.3390/nu9060621]
Patrocinador
European Union (EU) 679303Resumen
Regulation of lipid metabolism is essential for treatment and prevention of several chronic
diseases such as obesity, diabetes, and cardiovascular diseases, which are responsible for most
deaths worldwide. It has been demonstrated that the AMP-activated protein kinase (AMPK) has
a direct impact on lipid metabolism by modulating several downstream-signaling components.
The main objective of the present work was to evaluate the in vitro effect of a methanolic strawberry
extract on AMPK and its possible repercussion on lipid metabolism in human hepatocellular
carcinoma cells (HepG2). For such purpose, the lipid profile and the expression of proteins
metabolically related to AMPK were determined on cells lysates. The results demonstrated that
strawberry methanolic extract decreased total cholesterol, low-density lipoprotein (LDL)-cholesterol,
and triglycerides levels (up to 0.50-, 0.30-, and 0.40-fold, respectively) while it stimulated the
p-AMPK/AMPK expression (up to 3.06-fold), compared to the control. AMPK stimulation led
to the phosphorylation and consequent inactivation of acetyl coenzyme A carboxylase (ACC) and
inhibition of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), the major regulators of fatty
acids and cholesterol synthesis, respectively. Strawberry treatment also entailed a 4.34-, 2.37-, and
2.47-fold overexpression of LDL receptor, sirtuin 1 (Sirt1), and the peroxisome proliferator activated
receptor gamma coactivator 1-alpha (PGC-1α), respectively, compared to control. The observed
results were counteracted by treatment with compound C, an AMPK pharmacological inhibitor,
confirming that multiple effects of strawberries on lipid metabolism are mediated by the activation of
this protein.