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Antiproliferative and Pro-Apoptotic Effect of Uvaol in Human Hepatocarcinoma HepG2 Cells by Affecting G0/G1 Cell Cycle Arrest, ROS Production and AKT/PI3K Signaling Pathway
dc.contributor.author | Bonel Pérez, Gloria C. B | |
dc.contributor.author | Pérez Jiménez, Amalia | |
dc.contributor.author | Gris Cárdenas, Isabel | |
dc.contributor.author | Parra Pérez, Alberto M. | |
dc.contributor.author | Lupiáñez Cara, José Antonio | |
dc.contributor.author | Reyes Zurita, Fernando Jesús | |
dc.contributor.author | Rufino Palomares, Eva | |
dc.date.accessioned | 2020-11-13T08:51:00Z | |
dc.date.available | 2020-11-13T08:51:00Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Bonel-Pérez, Gloria C.; Pérez-Jiménez, Amalia; Gris-Cárdenas, Isabel; Parra-Pérez, Alberto M.; Lupiáñez, José A.; Reyes-Zurita, Fernando J.; Siles, Eva; Csuk, René; Peragón, Juan; Rufino-Palomares, Eva E. 2020. "Antiproliferative and Pro-Apoptotic Effect of Uvaol in Human Hepatocarcinoma HepG2 Cells by Affecting G0/G1 Cell Cycle Arrest, ROS Production and AKT/PI3K Signaling Pathway." Molecules 25, no. 18: 4254. [doi: 10.3390/molecules25184254] | es_ES |
dc.identifier.uri | http://hdl.handle.net/10481/64245 | |
dc.description.abstract | Natural products have a significant role in the development of new drugs, being relevant the pentacyclic triterpenes extracted from Olea europaea L. Anticancer effect of uvaol, a natural triterpene, has been scarcely studied. The aim of this study was to understand the anticancer mechanism of uvaol in the HepG2 cell line. Cytotoxicity results showed a selectivity effect of uvaol with higher influence in HepG2 than WRL68 cells used as control. Our results show that uvaol has a clear and selective anticancer activity in HepG2 cells supported by a significant anti-migratory capacity and a significant increase in the expression of HSP-60. Furthermore, the administration of this triterpene induces cell arrest in the G0/G1 phase, as well as an increase in the rate of cell apoptosis. These results are supported by a decrease in the expression of the anti-apoptotic protein Bcl2, an increase in the expression of the pro-apoptotic protein Bax, together with a down-regulation of the AKT/PI3K signaling pathway. A reduction in reactive oxygen species (ROS) levels in HepG2 cells was also observed. Altogether, results showed anti-proliferative and pro-apoptotic effect of uvaol on hepatocellular carcinoma, constituting an interesting challenge in the development of new treatments against this type of cancer. | es_ES |
dc.description.sponsorship | Research Plan, Action 7 of University of Jaen, Spain UJA2014/07/13 | es_ES |
dc.description.sponsorship | General Secretariat of Universities, Research and Technology of the Ministry of Economy, Innovation, Science and Employment | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | Atribución 3.0 España | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | AKT/PI3K signaling pathway | es_ES |
dc.subject | Apoptosis | es_ES |
dc.subject | Human hepatocarcinoma HepG2 cells | es_ES |
dc.subject | Migration activity | es_ES |
dc.subject | Proliferation | es_ES |
dc.subject | Oxidative stress | es_ES |
dc.subject | ROS levels | es_ES |
dc.subject | Uvaol | es_ES |
dc.title | Antiproliferative and Pro-Apoptotic Effect of Uvaol in Human Hepatocarcinoma HepG2 Cells by Affecting G0/G1 Cell Cycle Arrest, ROS Production and AKT/PI3K Signaling Pathway | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.doi | 10.3390/molecules25184254 |