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dc.contributor.authorSoriano-Lerma, Ana
dc.contributor.authorPérez‑Carrasco, Virginia
dc.contributor.authorSánchez Marañón, Manuel 
dc.contributor.authorOrtiz-González, Matilde
dc.contributor.authorSánchez‑Martín, Victoria
dc.contributor.authorGijón Martín, Juan José 
dc.contributor.authorNavarro-Marí, José María
dc.contributor.authorGarcía-Salcedo, José Antonio
dc.contributor.authorSoriano, Miguel
dc.identifier.citationSoriano-Lerma, A., Pérez-Carrasco, V., Sánchez-Marañón, M. et al. Influence of 16S rRNA target region on the outcome of microbiome studies in soil and saliva samples. Sci Rep 10, 13637 (2020). []es_ES
dc.descriptionThis work was financially supported by the Ministry of Economy and Competitiveness of Spain co-financed with European Regional Development Funds (References: CGL2015-71709-R and SAF2015-71714-R) and the fellowship PEJ2018-004702-A. The European Union also collaborated in the funding of this study (Euronanomed programme, Reference: TARBRAINFECT, and Innovative Medicines Initiative IMI2, Reference: 3TR-831434), as well as the Health Institute Carlos III as the local management institution (Reference: AC18/00008). A.S.L. and V.S.M. were supported by a fellowship from the Ministry of Education, Culture and Sport (FPU 17/05413 and FPU 16/05822), and the results presented in this article are part of A.S.L's doctoral thesis. M.O.G acknowledges for the funds received by the F.P.U. fellowship provided by University of Almeria.es_ES
dc.description.abstractNext generation sequencing methods are widely used in evaluating the structure and functioning of microbial communities, especially those centered on 16S rRNA subunit. Since Illumina Miseq, the most used sequencing platform, does not allow the full sequencing of 16S rRNA gene, this study aims to evaluate whether the choice of different target regions might affect the outcome of microbiome studies regarding soil and saliva samples. V1V3, V3V4, V4V5 and V6V8 domains were studied, finding that while some regions showed differences in the detection of certain bacterial taxa and in the calculation of alpha diversity, especially in soil samples, the overall effect did not compromise the differentiation of any sample type in terms of taxonomic analysis at the genus level. 16S rRNA target regions did affect the detection of specific bacteria related to soil quality and development, and microbial genera used as health biomarkers in saliva. V1V3 region showed the closest similarity to internal sequencing control mock community B, suggesting it might be the most preferable choice regarding data reliability.es_ES
dc.description.sponsorshipEuropean Union (EU) 3TR-831434es_ES
dc.description.sponsorshipInstituto de Salud Carlos III AC18/00008es_ES
dc.description.sponsorshipMinistry of Education, Culture and Sport FPU 17/05413 FPU 16/05822es_ES
dc.description.sponsorshipUniversity of Almeriaes_ES
dc.description.sponsorshipMinistry of Economy and Competitiveness of Spaines_ES
dc.description.sponsorshipEuropean Union (EU) CGL2015-71709-R SAF2015-71714-R PEJ2018-004702-Aes_ES
dc.publisherSpringer Naturees_ES
dc.rightsAtribución 3.0 España*
dc.subjectMicrobial communityes_ES
dc.subjectMicrobiology es_ES
dc.titleInfluence of 16S rRNA target region on the outcome of microbiome studies in soil and saliva sampleses_ES

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Except where otherwise noted, this item's license is described as Atribución 3.0 España