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dc.contributor.authorGallardo, Amador
dc.contributor.authorMolina, Aldara
dc.contributor.authorAsenjo, Helena G
dc.contributor.authorMartorell Marugán, Jordi 
dc.contributor.authorMontes, Rosa
dc.contributor.authorRamos Mejía, Verónica
dc.contributor.authorSánchez Pozo, Antonio 
dc.contributor.authorCarmona Sáez, Pedro 
dc.contributor.authorLópez Onieva, Lourdes 
dc.contributor.authorLandeira, David
dc.date.accessioned2020-11-04T11:12:42Z
dc.date.available2020-11-04T11:12:42Z
dc.date.issued2020-04-13
dc.identifier.citationGallardo, A., Molina, A., Asenjo, H. G., Martorell-Marugán, J., Montes, R., Ramos-Mejia, V., ... & Landeira, D. (2020). The molecular clock protein Bmal1 regulates cell differentiation in mouse embryonic stem cells. Life Science Alliance, 3(5). [http://doi.org/10.26508/lsa.201900535]es_ES
dc.identifier.urihttp://hdl.handle.net/10481/64045
dc.description.abstractMammals optimize their physiology to the light–dark cycle by synchronization of the master circadian clock in the brain with peripheral clocks in the rest of the tissues of the body. Circadian oscillations rely on a negative feedback loop exerted by the molecular clock that is composed by transcriptional activators Bmal1 and Clock, and their negative regulators Period and Cryptochrome. Components of the molecular clock are expressed during early development, but onset of robust circadian oscillations is only detected later during embryogenesis. Here, we have used na¨ıve pluripotent mouse embryonic stem cells (mESCs) to study the role of Bmal1 during early development. We found that, compared to wild-type cells, Bmal12/2 mESCs express higher levels of Nanog protein and altered expression of pluripotencyassociated signalling pathways. Importantly, Bmal12/2 mESCs display deficient multi-lineage cell differentiation capacity during the formation of teratomas and gastrula-like organoids. Overall, we reveal that Bmal1 regulates pluripotent cell differentiation and propose that the molecular clock is an hitherto unrecognized regulator of mammalian development.es_ES
dc.description.sponsorshipRamon y Cajal grant of the Spanish ministry of economy and competitiveness RYC2012-10019es_ES
dc.description.sponsorshipSpanish ministry of economy and competitiveness BFU2016-75233-Pes_ES
dc.description.sponsorshipAndalusian regional government PC-0246-2017es_ES
dc.description.sponsorshipFundacion Progreso y Salud (FPS)es_ES
dc.description.sponsorshipInstituto de Salud Carlos III European Union (EU) CPII17/00032 PI17/01574es_ES
dc.description.sponsorshipUniversity of Granadaes_ES
dc.language.isoenges_ES
dc.publisherLife Science Alliance LLCes_ES
dc.rightsAtribución 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.titleThe molecular clock protein Bmal1 regulates cell differentiation in mouse embryonic stem cellses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.doi10.26508/lsa.201900535
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones_ES


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Atribución 3.0 España
Except where otherwise noted, this item's license is described as Atribución 3.0 España