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dc.contributor.authorRivero Gutiérrez, Belén
dc.contributor.authorAnzola Santander, Andrea
dc.contributor.authorMartínez Augustín, María Olga 
dc.contributor.authorSánchez De Medina López-Huertas, Fermín
dc.identifier.citationRivero-Gutiérrez, B., Anzola, A., Martínez-Augustin, O., & de Medina, F. S. (2014). Stain-free detection as loading control alternative to Ponceau and housekeeping protein immunodetection in Western blotting. Analytical biochemistry, 467, 1-3. []es_ES
dc.descriptionThe authors want to thank the input of Arnaud Remy and Enrique Orozco of Bio-Rad Laboratories. This study was supported by the Ministry of Science and Innovation (SAF2011-22922 and SAF2011-22812), and by funds from Junta de Andalucia (CTS6736 and CTS164). B. Rivero and A. Anzola were supported by fellowships of the Ministry of Education and Science of Spain and Junta de Andalucia, respectively. CIBERehd is funded by the Instituto de Salud Carlos III.es_ES
dc.description.abstractIt is currently a routine practice to require a measurement of a housekeeping reference, including actin, glyceraldehyde-3-phosphate dehydrogenase, β-tubulin, among others, in Western blots, as it is the rule in RNA blots. Reversible Ponceau staining has been applied successfully to check equal loading of gels. Here we test a new technique, with the Stain-Free gels from Bio-Rad, against both Ponceau staining and housekeeping protein immunodetection under different conditions. Our results show that Stain-Free gels outperform Ponceau staining and that both are more consistent than housekeeping proteins as a loading control.es_ES
dc.description.sponsorshipSpanish Government SAF2011-22922 SAF2011-22812es_ES
dc.description.sponsorshipJunta de Andalucia CTS6736 CTS164es_ES
dc.description.sponsorshipInstituto de Salud Carlos IIIes_ES
dc.rightsAtribución 3.0 España*
dc.subjectWestern blotes_ES
dc.subjectLoading controles_ES
dc.subjectPonceau staininges_ES
dc.subjectHousekeeping proteinses_ES
dc.subjectTGX Stain-Free gelses_ES
dc.titleStain-free detection as loading control alternative to Ponceau and housekeeping protein immunodetection in Western blottinges_ES

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Atribución 3.0 España
Except where otherwise noted, this item's license is described as Atribución 3.0 España