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dc.contributor.authorPadial Molina, Miguel 
dc.contributor.authorBuitrago, Juan G. de
dc.contributor.authorSainz-Urruela, Raquel
dc.contributor.authorAbril García, Darío
dc.contributor.authorAnderson, Per
dc.contributor.authorO'Valle Ravassa, Francisco Javier 
dc.contributor.authorGalindo Moreno, Pablo Antonio
dc.identifier.citationPadial-Molina, M.; de Buitrago, J.G.; Sainz-Urruela, R.; Abril-Garcia, D.; Anderson, P.; O’Valle, F.; Galindo-Moreno, P. Expression of Musashi-1 During Osteogenic Differentiation of Oral MSC: An In Vitro Study. Int. J. Mol. Sci. 2019, 20, 2171. [doi:10.3390/ijms20092171]es_ES
dc.descriptionSupplementary materials can be found at
dc.description.abstractBackground: Musashi-1 (MSI1) is a negative regulator of mesenchymal stromal cell (MSC) differentiation which in turn favors cell proliferation. However, little is known about its expression by MSC from the oral cavity and in the context of osteogenic differentiation. Aim: The aim of this study was to analyze the expression of MSI1 in the context of osteogenic differentiation of MSC derived from the oral cavity. Material/methods: For this in vitro study, MSC were isolated from six different origins of the oral cavity. They were extensively characterized in terms of proliferative and clonogenicity potential, expression of stemness genes (MYC, NANOG, POU5F1, and SOX2), expression of surface markers (CD73, CD90, CD105, CD14, CD31, CD34, and CD45) and adipo-, chondro- and osteogenic differentiation potential. Then, osteogenic differentiation was induced and the expression of MSI1 mRNA and other relevant markers of osteogenic differentiation, including RUNX2 and Periostin, were also evaluated. Results: Cell populations from the alveolar bone (pristine or previously grafted with xenograft), dental follicle, dental germ, dental pulp, and periodontal ligament were obtained. The analysis of proliferative and clonogenicity potential, expression of the stemness genes, expression of surface markers, and differentiation potential showed similar characteristics to those of previously published MSC from the umbilical cord. Under osteogenic differentiation conditions, all MSC populations formed calcium deposits and expressed higher SPARC. Over time, the expression of MSI1 followed different patterns for the different MSC populations. It was not significantly different than the expression of RUNX2. In contrast, the expression of MSI1 and POSTN and RUNX2 were statistically different in most MSC populations. Conclusion: In the current study, a similar expression pattern of MSI1 and RUNX2 during in vitro osteogenic differentiation was identified.es_ES
dc.description.sponsorshipThe authors of this investigation were partially supported by Research Groups #CTS-138 (F.O.) and #CTS-1028 (M.P.-M., P.G.-M.) (Junta de Andalucía, Spain), a grant from MIS Implant Technologies Ltd. (M.P.-M., D.A.-G., P.G.-M.), the Youth Employment Initiative (YEI) from the European Commission (R.S.-U.), and the Instituto de Salud Carlos III, Spain ( and Fondo Europeo de Desarrollo Regional (FEDER, from the European Union), through the research grants PI15/00794 and CPII15/00032 (P.A).es_ES
dc.rightsAtribución 3.0 España*
dc.subjectMesenchymal stromal cellses_ES
dc.subjectOsteogenic differentiationes_ES
dc.subjectbone regenerationes_ES
dc.subjectBone healinges_ES
dc.titleExpression of Musashi-1 During Osteogenic Differentiation of Oral MSC: An In Vitro Studyes_ES

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Atribución 3.0 España
Except where otherwise noted, this item's license is described as Atribución 3.0 España