The Secretion of Streptomyces monbaraensis Transglutaminase From Lactococcus lactis and Immobilization on Porous Magnetic Nanoparticles
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Frontiers in Media
Materia
Transglutaminase Lactococcus lactis Signal peptide SPusp45 Immobilized enzyme
Date
2019-08-06Referencia bibliográfica
Ma T, Lu J, Zhu J, Li X, Gu H, Montalbán-López M, Wu X, Luo S, Zhao Y, Jiang S, Zheng Z and Mu D (2019) The Secretion of Streptomyces monbaraensis Transglutaminase From Lactococcus lactis and Immobilization on Porous Magnetic Nanoparticles. Front. Microbiol. 10:1675.
Sponsorship
This study was granted by the National Key Research and Development Program of China (2018YFD0400600 and 2018YFD0400400), Key Scientific and Technological Project of Anhui Province of China (Nos. 17030701014 and 18030701146), Anhui Provincial Natural Science Foundation (1708085QC65), the Open Fund of State Key Laboratory of Tea Plant Biology and Utilization (SKLTOF20180107), and China Postdoctoral Science Foundation (2019M651013).Abstract
Microbial transglutaminase (MTG) from Streptomyces mobaraensis is an important
enzyme widely applied in food processing for the improvement of protein properties
by catalyzing the cross-linking of proteins. In this work we aimed at improving
the production and enabling an easy and efficient purification process from culture
supernatants. Thus, recombinant vectors, with either a constitutive promoter (Pp5) or
an inducible promoter (PnisA), controlling the expression of the MTG gene fused to the
signal peptide of Usp45 (SPusp45) were constructed and then expressed in Lactococcus
lactis. After purification, 43.5 +/- 0.4 mg/L mature MTG-6His was obtained. It displayed
27.6 +/- 0.5 U/mg enzymatic activity cross-linking soy protein isolate effectively. The
purified mature MTG was immobilized with magnetic porous Fe3O4 nanoparticles,
which improved its activity up to 29.1 +/- 0.4 U/mg. The immobilized MTG maintained
67.2% of the initial activity after being recycled for 10 times. The high production
and secretion of functional S. mobaraensis MTG from L. lactis and the magnetic
immobilized MTG-6His onto Fe3O4 nanoparticles reported in this study would have
potential industrial applications.