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dc.contributor.authorCianciosi, Danila
dc.contributor.authorQuiles Morales, José Luis 
dc.date.accessioned2020-03-04T12:05:22Z
dc.date.available2020-03-04T12:05:22Z
dc.date.issued2020-01-10
dc.identifier.citationCianciosi, D., Forbes-Hernández, T. Y., Afrin, S., Gasparrini, M., Quiles, J. L., Gil, E., ... & Giampieri, F. (2020). The Influence of In Vitro Gastrointestinal Digestion on the Anticancer Activity of Manuka Honey. Antioxidants, 9(1), 64.es_ES
dc.identifier.urihttp://hdl.handle.net/10481/60002
dc.description.abstractManuka honey (MH) is a natural food with many beneficial properties to human health, thanks to its high variety of bioactive compounds; however, little is known about its bioaccessibility. The aim of this study was to evaluate and compare the polyphenol compounds, the antioxidant capacity and the anticancer activity of MH subjected to an in vitro gastrointestinal digestion in human HCT-116 colon cancer cells. Raw MH and digested MH (DMH) were assessed for total polyphenols and flavonoids by spectrophotometric and HPLC-ESI-MS/MS analysis, and total antioxidant capacity (TAC) using different methods. Cell viability, intracellular ROS production, apoptosis, cell cycle and colony formation capacity were tested after treatment with MH or DMH. Results showed that total polyphenols, total flavonoids and TAC were significantly (p < 0.05) reduced after in vitro digestion. In addition, MH and DMH at 8, 16 and 24 mg/mL had similar effects in inducing intracellular ROS production and in inhibiting the colon formation ability; MH induced a more marked apoptosis compared to DMH, while cell cycle was blocked in S phase by MH and in Sub G1 phase by DMH. Our results increase knowledge of the effect of gastrointestinal digestion on the biological effect of honey against colorectal cancer.es_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightsAtribución 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjectBioaccessibilityes_ES
dc.subjectBioavailabilityes_ES
dc.subjectColon canceres_ES
dc.subjectHoneybee es_ES
dc.subjectIn vitro simulated digestiones_ES
dc.titleThe Influence of In Vitro Gastrointestinal Digestion on the Anticancer Activity of Manuka Honeyes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.doi10.3390/antiox9010064


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Atribución 3.0 España
Except where otherwise noted, this item's license is described as Atribución 3.0 España