Paenibacillus lutrae sp. nov., A Chitinolytic Species Isolated from A River Otter in Castril Natural Park, Granada, Spain
Metadatos
Mostrar el registro completo del ítemAutor
Rodríguez, Miguel; Reina, José Carlos; Béjar Luque, María Victoria; Llamas Company, InmaculadaEditorial
MDPI
Materia
Paenibacillus lutrae River otter New species Chitinolytic activity
Fecha
2019-12-02Referencia bibliográfica
Rodríguez, M., Reina, J. C., Béjar, V., & Llamas, I. (2019). Paenibacillus lutrae sp. nov., A Chitinolytic Species Isolated from A River Otter in Castril Natural Park, Granada, Spain. Microorganisms, 7(12), 637.
Patrocinador
This study was supported by a grant from the Spanish Ministerio de Educación y Ciencia (AGL-2015-68806-R) and from the Plan Andaluz de Investigación (Research Group BIO 188). Miguel A. Rodríguez is supported by a University of Granada Programme (Empleo Garantía Juvenil). José C. Reina is supported by an FPU fellowship from the Spanish Ministerio de Educación, Cultura y Deporte (FPU15/01717).Resumen
A highly chitinolytic facultative anaerobic, chemoheterotrophic, endospore-forming,
Gram-stain-positive, rod-shaped bacterial strain N10T was isolated from the feces of a river otter in
the Castril Natural Park (Granada, Spain). It is a slightly halophilic, motile, catalase-, oxidase-, ACC
deaminase- and C4 and C8 lipase-positive strain. It is aerobic, respiratory and has a fermentative
metabolism using oxygen as an electron acceptor, produces acids from glucose and can fix nitrogen.
Phylogenetic analysis of the 16S rRNA gene sequence, multilocus sequence analysis (MLSA) of
16S rRNA, gyrB, recA and rpoB, as well as phylogenomic analyses indicate that strain N10T is a
novel species of the genus Paenibacillus, with the highest 16S rRNA sequence similarity (95.4%) to
P. chitinolyticus LMG 18047T and <95% similarity to other species of the genus Paenibacillus. Digital
DNA–DNA hybridization (dDDH) and average nucleotide identity (ANIb) were 21.1% and <75%,
respectively. Its major cellular fatty acids were anteiso-C15:0, C16:0, and iso-C15:0. G + C content ranged
between 45%–50%. Using 16S rRNA phylogenetic and in silico phylogenomic analyses, together with
chemotaxonomic and phenotypic data, we demonstrate that type strain N10T (= CECT 9541T =LMG
30535T) is a novel species of genus Paenibacillus and the name Paenibacillus lutrae sp. nov. is proposed.