Mostrar el registro sencillo del ítem

dc.contributor.authorToledano Pérez, Manuel 
dc.contributor.authorToledano Osorio, Manuel 
dc.contributor.authorNavarro Hortal, María Dolores 
dc.contributor.authorVarela López, Alfonso 
dc.contributor.authorOsorio Ruiz, Raquel 
dc.contributor.authorQuiles Morales, José Luis 
dc.date.accessioned2020-01-09T13:47:49Z
dc.date.available2020-01-09T13:47:49Z
dc.date.issued2019-11-14
dc.identifier.citationToledano, M., Toledano-Osorio, M., Navarro-Hortal, M. D., Varela-López, A., Osorio, R., & Quiles, J. L. (2019). Novel Polymeric Nanocarriers Reduced Zinc and Doxycycline Toxicity in the Nematode Caenorhabditis elegans. Antioxidants, 8(11), 550.es_ES
dc.identifier.urihttp://hdl.handle.net/10481/58609
dc.description.abstractThe objective was to evaluate the toxicity of zinc- and doxycycline-loaded polymeric nanoparticles (NPs) using Caenorhabditis elegans as a model organism. These NPs are composed of ethylene glycol dimethacrylate, 2-hydroxyethyl methacrylate and methacrylic acid. NPs were loaded with doxycycline (D-NPs) and zinc (Zn-NPs) by chemical adsorption, and loading efficacy was demonstrated. Worm death rate in a concentration-response curve basis was calculated for lethality. Metabolism was evaluated through pharyngeal pumping assay. Body length measurements, brood size and egg lays were used to gauge growth, reproduction and fertility respectively. Intracellular hydrogen peroxide levels were determined to assess the reactive oxygen species production. One-way ANOVA and Bonferroni were used for comparisons (p < 0.05). Tested NPs at the highest dosage did not affect lethality or worm metabolism, expressed in terms of death rate and pharyngeal pumping per minute, respectively. Zn-NPs slightly increased worm growth. The concentration of the intracellular hydrogen peroxide levels was the lowest in the D-NPs group. The distinct NPs and concentrations employed were shown to be non-toxic for in situ administration of zinc and doxycycline, reducing the harmful effects of these compounds.es_ES
dc.description.sponsorshipThis research was funded by the Ministry of Economy and Competitiveness and European Regional Development Fund (MINECO/FEDER MAT2017-85999P), and by the University of Granada (Excellence Program UNETE/UCE-PP2017-06).es_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightsAtribución 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjectDentistry es_ES
dc.subjectDoxycyclinees_ES
dc.subjectFertility es_ES
dc.subjectGrowth es_ES
dc.subjectLethalityes_ES
dc.subjectMetabolism es_ES
dc.subjectNanoparticleses_ES
dc.titleNovel Polymeric Nanocarriers Reduced Zinc and Doxycycline Toxicity in the Nematode Caenorhabditis eleganses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.doi10.3390/antiox8110550


Ficheros en el ítem

[PDF]

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem

Atribución 3.0 España
Excepto si se señala otra cosa, la licencia del ítem se describe como Atribución 3.0 España