A versatile theranostic nanodevice based on an orthogonal bioconjugation strategy for efficient targeted treatment and monitoring of triple negative breast cancer
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AuthorCano Cortes, María Victoria; Navarro Marchal, Saul Abenhamar; Ruiz-Blas, María Paz; Díaz Mochón, Juan José; Marchal Corrales, Juan Antonio; Sánchez Martín, Rosario María
Polymeric nanoparticlesOrthogonal chemistryCovalent conjugationDoxorubicinCRGDK targetingMultifunctional theranostic nanodevice
Cano-Cortes M.V., et al., A versatile theranostic nanodevice based on an orthogonal bioconjugation strategy for efficient targeted treatment and monitoring .... Nanomedicine: NBM 2020;24:1-11, [https://doi.org/10.1016/j.nano.2019.102120]
SponsorshipMinistry of Economy and Competitiveness (MINECO, National Programme for Research Aimed at the Challenges of Society, project reference BIO2016-80519-R&FEDER funds, grant number MAT2015-62644.C2.2.R); Ministerio de Ciencia, Innovación y Universidades grant number RTI2018-101309-B-C2 (FEDER Funds); Health Institute Carlos III (ISCIII) (Projects of technological development in health - ref DTS18/00121); University of Granada – PLAN OF RESEARCH AND TRANSFER 2016 –PSETC- PSE/16/003); Project MAT2015-62644.C2.2.R
A novel chemical-based orthogonal bioconjugation strategy to produce tri-functionalized nanoparticles (NPs) an chemotherapy drug, doxorubicin (DOX), a near-infrared cyanine dye (Cy7) and CRGDK homing peptide, a peptide specifically binds to neuropilin-1 (Nrp-1) overexpressed on triple negative breast cancer (TNBC) cells, has been validated. These theranostic NPs have been evaluated in vitro and in vivo using an orthotopic xenotransplant mouse model using TNBC cells. In vitro assays show that theranostic NPs improve the therapeutic index in comparison with free DOX. Remarkably, in vivo studies showed preferred location of theranostic NPs in the tumor area reducing the volume at the same level than free DOX while presenting lower side effects. This multifunctionalized theranostic nanodevice based on orthogonal conjugation strategies could be a good candidate for the treatment and monitoring of Nrp-1 overexpressing tumors. Moreover, this versatile nanodevice can be easily adapted to treat and monitor different cancer types by adapting the conjugation strategy.