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dc.contributor.authorManzano Moreno, Francisco Javier
dc.contributor.authorIllescas Montes, Rebeca
dc.contributor.authorMelguizo Rodríguez, Lucía Raquel 
dc.contributor.authorCostela Ruíz, Víctor
dc.contributor.authorGarcía Martínez, Olga 
dc.contributor.authorRuiz Rodríguez, Concepción 
dc.contributor.authorRamos Torrecillas, Javier 
dc.date.accessioned2019-12-12T10:42:06Z
dc.date.available2019-12-12T10:42:06Z
dc.date.issued2019-10-21
dc.identifier.citationManzano, F. J. (2019). Impact of bisphosphonates on the proliferation and gene expression of human fibroblasts. International Journal of Medical Sciences, 16(12), 1534-1540.es_ES
dc.identifier.urihttp://hdl.handle.net/10481/58272
dc.description.abstractThe aim of this study was to elucidate the role of fibroblasts in bisphosphonate-related osteonecrosis of the jaw (BRONJ), evaluating the effect of zoledronate, alendronate, and ibandronate on the proliferation of fibroblasts and on their expression of genes essential for fibroblast physiology. Human CCD-1064Sk epithelial fibroblast cells were incubated in culture medium with 10-5, 10-7, or 10-9 M zoledronate, alendronate, or ibandronate. The proliferative capacity of fibroblasts was determined by spectrophotometry (MTT) at 24 of culture. Real-time polymerase chain reaction (RT-PCR) was used to study the effects of BPs at a dose of 10-9 M on the expression of FGF, CTGF, TGF-β1, TGFβR1, TGFβR2, TGFβR3, DDR2, α-actin, fibronectin, decorin, and elastin. Fibroblasts proliferation was significantly increased at the lowest dose (10-9M) of each BP but was not affected at the higher doses (10-5 and 10-7M). The proliferation increase may be related to the rise in TGF-β1 and TGFβR1 expression detected after the treatment of cells with 10-9M of zoledronate, alendronate, or ibandronate. However, the expression of CTGF, DDR2, α-actin, fibronectin, and decorin decreased versus controls. The results of this in vitro study indicate that a very low BP dose (10-9 M) can significantly affect the physiology of fibroblasts, increasing their proliferative capacity and modulating the expression of multiple genes involved in their growth and differentiation.es_ES
dc.language.isoenges_ES
dc.publisherIvyspring International Publisheres_ES
dc.rightsAtribución 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjectBisphosphonateses_ES
dc.subjectOsteonecrosises_ES
dc.subjectFibroblastes_ES
dc.subjectGene expressiones_ES
dc.titleImpact of bisphosphonates on the proliferation and gene expression of human fibroblastses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.doi10.7150/ijms.36994


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Atribución 3.0 España
Except where otherwise noted, this item's license is described as Atribución 3.0 España