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dc.contributor.authorMariotto, Elena
dc.contributor.authorGiampietro, Viola
dc.contributor.authorRonca, Roberto
dc.contributor.authorPersano, Luca
dc.contributor.authorAveic, Sanja
dc.contributor.authorBhujwalla, Zaver M.
dc.contributor.authorMori, Noriko
dc.contributor.authorAccordi, Benedetta
dc.contributor.authorSerafin, Valentina
dc.contributor.authorLópez Cara, Luisa Carlota 
dc.contributor.authorBortolozzi, Roberta
dc.identifier.citationMariotto, El. [et al.]. Choline Kinase Alpha Inhibition by EB-3D Triggers Cellular Senescence, Reduces Tumor Growth and Metastatic Dissemination in Breast Cancer.Cancers 2018, 10, 391; doi:10.3390/cancers10100391.es_ES
dc.description.abstractCholine kinase (ChoK) is the first enzyme of the Kennedy pathway leading to the biosynthesis of phosphatidylcholine (PtdCho), the most abundant phospholipid in eukaryotic cell membranes. EB-3D is a novel choline kinase 1 (ChoK 1) inhibitor with potent antiproliferative activity against a panel of several cancer cell lines. ChoK 1 is particularly overexpressed and hyperactivated in aggressive breast cancer. By NMR analysis, we demonstrated that EB-3D is able to reduce the synthesis of phosphocholine, and using flow cytometry, immunoblotting, and q-RT-PCR as well as proliferation and invasion assays, we proved that EB-3D strongly impairs breast cancer cell proliferation, migration, and invasion. EB-3D induces senescence in breast cancer cell lines through the activation of the metabolic sensor AMPK and the subsequent dephosphorylation of mTORC1 downstream targets, such as p70S6K, S6 ribosomal protein, and 4E-BP1. Moreover, EB-3D strongly synergizes with drugs commonly used for breast cancer treatment. The antitumorigenic potential of EB-3D was evaluated in vivo in the syngeneic orthotopic E0771 mouse model of breast cancer, where it induces a significant reduction of the tumor mass at low doses. In addition, EB-3D showed an antimetastatic effect in experimental and spontaneous metastasis models. Altogether, our results indicate that EB-3D could be a promising new anticancer agent to improve aggressive breast cancer treatment protocols.es_ES
dc.description.sponsorshipThis work was supported by funds from Istituto di Ricerca Pediatrica (IRP)-Città della Speranza and Cassa di Risparmio di Padova e Rovigo—CARIPARO Foundation (project IRP13/05) and by the University of Granada, (Cei-Biotic project CEI2013-MP-1), and Associazione Italiana per la Ricerca sul Cancro (AIRC) MFAG 18459 grant (R.R.). E.M. was supported by AIRC (21101) and V.S. by FIRC (16616) fellowships.es_ES
dc.rightsAtribución 3.0 España*
dc.subjectCholine Kinase αes_ES
dc.subjectBreast canceres_ES
dc.subjectSmall moleculeses_ES
dc.titleCholine Kinase Alpha Inhibition by EB-3D Triggers Cellular Senescence, Reduces Tumor Growth and Metastatic Dissemination in Breast Canceres_ES

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Atribución 3.0 España
Except where otherwise noted, this item's license is described as Atribución 3.0 España