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dc.contributor.authorHackenberg , Michael es_ES
dc.contributor.authorRueda, Antonioes_ES
dc.contributor.authorGustafson, Perryes_ES
dc.contributor.authorLangridge, Peteres_ES
dc.contributor.authorShi, Bu-Junes_ES
dc.date.accessioned2018-03-07T10:15:43Z
dc.date.available2018-03-07T10:15:43Z
dc.date.issued2016
dc.identifier.citationHackenberg, M.; et al. Generation of different sizes and classes of small RNAs in barley is locus, chromosome and/or cultivar-dependent. BMC Genomics, 17: 735 (2016). [http://hdl.handle.net/10481/49876]es_ES
dc.identifier.issn1471-2164
dc.identifier.urihttp://hdl.handle.net/10481/49876
dc.description.abstractBackground: Various small RNA (sRNA) sizes and varieties have been identified, but their relationship as well as relationship with their origins and allocations have not been well understood or investigated. Results: By comparing sRNAs generated from two barley cultivars, Golden Promise (GP) and Pallas, we identified that the generation of different sizes and types of sRNAs in barley was locus-, chromosome- and/or cultivar-dependent. 20-nt sRNAs mainly comprising miRNAs and chloroplast-derived sRNAs were significantly over-expressed in Pallas vs. GP on chromosomes 3H and 6H. MiRNAs-enriched 21-nt sRNAs were significantly over-expressed in Pallas vs. GP only on chromosome 4H. On chromosome 5H this size of sRNAs was significantly under-expressed in Pallas, so were 22-nt sRNAs mainly comprising miRNAs and repeat-derived sRNAs. 24-nt sRNAs mostly derived from repeats were evenly distributed in all chromosomes and expressed similarly between GP and Pallas. Unlike other sizes of sRNAs, 24-nt sRNAs were little conserved in other plant species. Abundant sRNAs were mostly generated from 3’ terminal regions of chromosome 1H and 5’ terminal regions of chromosome 5H. Over-expressed miRNAs in GP vs. Pallas primarily function in stress responses and iron-binding. Conclusions: Our study indicates that 23−24-nt sRNAs may be linked to repressive chromatin modifications and function in genome stability while 20−21-nt sRNAs may be important for the cultivar specificity. This study provides a novel insight into the mechanism of sRNA expression and function in barley.en_EN
dc.description.sponsorshipThis work was funded by the Australian Research Council, the Grains Research and Development Corporation and the South Australian Government. None of these funding bodies were involved in the design of the study nor the collection, analysis, and interpretation of data and in writing the manuscript.es_ES
dc.language.isoenges_ES
dc.publisherBiomed Centrales_ES
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 3.0 Licensees_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es_ES
dc.subjectSmall RNA expressionen_EN
dc.subjectOriginen_EN
dc.subjectChromosome locationen_EN
dc.subjectConservation en_EN
dc.subjectBarley cultivaren_EN
dc.titleGeneration of different sizes and classes of small RNAs in barley is locus, chromosome and/or cultivar-dependenten_EN
dc.typejournal articlees_ES
dc.rights.accessRightsopen accesses_ES
dc.identifier.doi10.1186/s12864-016-3023-5


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