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McCLEC, a robust and stable enzymatic based microreactor platform

dc.contributor.authorConejero-Muriel, Maytees_ES
dc.contributor.authorRodríguez-Ruiz, Isaaces_ES
dc.contributor.authorMartínez-Ruiz, Sergioes_ES
dc.contributor.authorLlobera, Andreues_ES
dc.contributor.authorGavira Gallardo, José Antonio es_ES
dc.date.accessioned2017-09-22T09:37:07Z
dc.date.available2017-09-22T09:37:07Z
dc.date.issued2015-08-20
dc.identifier.citationConejero-Muriel, M.; et al. McCLEC, a robust and stable enzymatic based microreactor platform. Lab on a Chip, 15: 4083-4089 (2015). [http://hdl.handle.net/10481/47518]es_ES
dc.identifier.issn1473-0197
dc.identifier.urihttp://hdl.handle.net/10481/47518
dc.description.abstractA microfluidic chip for cross-linked enzyme crystals (McCLEC) is presented and demonstrated to be a stable, reusable and robust biocatalyst-based device with very promising biotechnological applications. The cost-effective microfluidic platform allows in situ crystallization, cross-linking and enzymatic reaction assays on a single device. A large number of enzymatic reuses of the McCLEC platform were achieved and a comparative analysis is shown illustrating the efficiency of the process and its storage stability for more than one year.en_EN
dc.description.sponsorshipThis work has been partly funded by the MICINN (Spain) projects BIO2010-16800 (JAG) and BIO2012-34937 (SMR), the European Commission (Contract No. 317916) under the LiPhos project (AL and IRR), “Factoría Española de Cristalización” Consolider-Ingenio 2010 (JAG and MCM) and EDRF Funds (JAG and AL). MCM thanks the Consejo Superior de Investigaciones Científicas (CSIC, Spain) for a JAE predoc fellowship.en_EN
dc.language.isoenges_ES
dc.publisherRoyal Society of Chemistryes_ES
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 3.0 Licensees_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es_ES
dc.subjectMicrofluidicen_EN
dc.subjectCrystallization en_EN
dc.subjectDeviceen_EN
dc.subjectMcCLECen_EN
dc.subjectEnzyme Assayen_EN
dc.titleMcCLEC, a robust and stable enzymatic based microreactor platformen_EN
dc.typejournal articlees_ES
dc.rights.accessRightsopen accesses_ES
dc.identifier.doi10.1039/c5lc00776c


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