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A zinc chloride-doped adhesive facilitates sealing at the dentin interface: A confocal laser microscopy study
dc.contributor.author | Toledano Pérez, Manuel | es_ES |
dc.contributor.author | Osorio Ruiz, Raquel | es_ES |
dc.contributor.author | Osorio Ruiz, María Estrella | es_ES |
dc.contributor.author | Cabello Malagón, Inmaculada | es_ES |
dc.contributor.author | Toledano Osorio, Manuel | es_ES |
dc.contributor.author | Sánchez Aguilera, Fátima | es_ES |
dc.date.accessioned | 2017-06-09T06:22:50Z | |
dc.date.available | 2017-06-09T06:22:50Z | |
dc.date.issued | 2017-05 | |
dc.identifier.citation | Toledano Pérez, M.; et al. A zinc chloride-doped adhesive facilitates sealing at the dentin interface: A confocal laser microscopy study. Journal of the Mechanical Behavior of Biomedical Materials, 74: 35-42 (2017). [http://hdl.handle.net/10481/46754] | es_ES |
dc.identifier.issn | 1751-6161 | |
dc.identifier.uri | http://hdl.handle.net/10481/46754 | |
dc.description.abstract | The aim of this study was to understand the effect of Zn-doping of adhesives and mechanical load cycling on the micromorphology of the resin-dentin interdiffusion zone (of sound and caries affected dentin). The investigation considered two different Zn-doped adhesive approaches and evaluated the interface using confocal laser scanning microscopy. Sound and carious dentin-resin interfaces of unloaded specimens were deficiently resin-hybridized, in general. These samples showed a rhodamine B-labeled hybrid layer and adhesive layer completely affected by fluorescein penetration (nanoleakage) through the porous resin-dentin interface, but thicker after phosphoric acid-etching and more extended in carious dentin. Zn-doping promoted an improved sealing of the resin-dentin interface at dentin, a decrease of the hybrid layer porosity, and an increment of dentin mineralization. Load cycled augmented the sealing of the Zn-doped resin-dentin interfaces, as porosity and nanoleakage diminished, and even disappeared in caries-affected dentin substrata conditioned with EDTA. Sound and carious dentin specimens treated with the xylenol orange technique produced a clearly outlined fluorescence when resins were Zn-doped, due to a consistent Ca-mineral deposits within the bonding interface and inside the dentinal tubules, especially when load cycling was applied on specimens treated with Zn-doped bonding components of self-etching adhesives. Micropermeability at the resin-dentin interface diminished after combining EDTA pretreatment, ZnCl2-doping and mechanical loading stimuli on restorations. It is clearly preferable to include the zinc compounds into the bonding constituents of the self-etching adhesives, instead of into the primer ingredients. The promoted new mineral segments contributed to reduce or avoid both porosity and nanoleakage from the load cycled Zn-doped resin dentin interface. | en_EN |
dc.description.sponsorship | This work was supported by the Ministry of Economy and Competitiveness (MINECO) [Project MAT2014-52036-P] and European Regional Development Fund (FEDER). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | |
dc.subject | Dentin | en_EN |
dc.subject | Load cycling | en_EN |
dc.subject | Confocal microscopy | en_EN |
dc.subject | Zn | en_EN |
dc.subject | Adhesive | en_EN |
dc.title | A zinc chloride-doped adhesive facilitates sealing at the dentin interface: A confocal laser microscopy study | en_EN |
dc.type | preprint | en_EN |
dc.rights.accessRights | open access | en_EN |
dc.identifier.doi | 10.1016/j.jmbbm.2017.04.030 |