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dc.contributor.authorAlfonso-Rodríguez, Camilo Andrés
dc.contributor.authorGarzón Bello, Ingrid Johanna 
dc.contributor.authorGarrido-Gómez, Juan
dc.contributor.authorXimenes Oliveira, Ana Celeste
dc.contributor.authorMartín Piedra, Miguel Ángel 
dc.contributor.authorScionti, Giuseppe
dc.contributor.authorCarriel Araya, Víctor 
dc.contributor.authorHernández-Cortés, Pedro
dc.contributor.authorCampos Muñoz, Antonio Jesús 
dc.contributor.authorAlaminos Mingorance, Miguel
dc.identifier.citationAlfonso-Rodríguez, C.A.; et al. Identification of Histological Patterns in Clinically Affected and Unaffected Palm Regions in Dupuytren's Disease. Plos One, 9(11): e112457 (2014). []es_ES
dc.description.abstractDupuytren's disease is a fibro-proliferative disease characterized by a disorder of the extracellular matrix (ECM) and high myofibroblast proliferation. However, studies failed to determine if the whole palm fascia is affected by the disease. The objective of this study was to analyze several components of the extracellular matrix of three types of tissues—Dupuytren's diseased contracture cords (DDC), palmar fascia clinically unaffected by Dupuytren's disease contracture (NPF), and normal forehand fascia (NFF). Histological analysis, quantification of cells recultured from each type of tissue, mRNA microarrays and immunohistochemistry for smooth muscle actin (SMA), fibrillar ECM components and non-fibrillar ECM components were carried out. The results showed that DDC samples had abundant fibrosis with reticular fibers and few elastic fibers, high cell proliferation and myofibroblasts, laminin and glycoproteins, whereas NFF did not show any of these findings. Interestingly, NPF tissues had more cells showing myofibroblasts differentiation and more collagen and reticular fibers, laminin and glycoproteins than NFF, although at lower level than DDC, with similar elastic fibers than DDC. Immunohistochemical expression of decorin was high in DDC, whereas versican was highly expressed NFF, with no differences for aggrecan. Cluster analysis revealed that the global expression profile of NPF was very similar to DDC, and reculturing methods showed that cells corresponding to DDC tissues proliferated more actively than NPF, and NPF more actively than NFF. All these results suggest that NPF tissues may be affected, and that a modification of the therapeutic approach used for the treatment of Dupuytren's disease should be considered.es_ES
dc.description.sponsorshipThis work was supported by CTS-115 (Tissue Engineering Group), University of Granada/Spain.es_ES
dc.publisherPublic Library of Science (PLOS)es_ES
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 3.0 License
dc.subjectSulfates es_ES
dc.subjectGene expressiones_ES
dc.subjectGlycoproteins es_ES
dc.subjectImmunohistochemical analysises_ES
dc.titleIdentification of Histological Patterns in Clinically Affected and Unaffected Palm Regions in Dupuytren's Diseasees_ES

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