dc.contributor.author | Giampieri, Francesca | es_ES |
dc.contributor.author | Álvarez-Suárez, José M. | es_ES |
dc.contributor.author | Mazzoni, Luca | es_ES |
dc.contributor.author | Forbes Hernández, Tamara Yuliett | es_ES |
dc.contributor.author | Gasparrini, Massimiliano | es_ES |
dc.contributor.author | González-Paramás, Ana M. | es_ES |
dc.contributor.author | Santos-Buelga, Celestino | es_ES |
dc.contributor.author | Quiles Morales, José Luis | es_ES |
dc.contributor.author | Bompadre, Stefano | es_ES |
dc.contributor.author | Mezzetti, Bruno | es_ES |
dc.contributor.author | Battino, Maurizio | es_ES |
dc.date.accessioned | 2014-09-03T11:45:17Z | |
dc.date.available | 2014-09-03T11:45:17Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | Giampieri, F.; et al. Polyphenol-Rich Strawberry Extract Protects Human Dermal Fibroblasts against Hydrogen Peroxide Oxidative Damage and Improves Mitochondrial Functionality. Molecules, 19(6): 7798-7816 (2014). [doi: 10.3390/molecules19067798] | es_ES |
dc.identifier.issn | 1420-3049 | |
dc.identifier.uri | http://hdl.handle.net/10481/32890 | |
dc.description.abstract | Strawberry bioactive compounds are widely known to be powerful antioxidants. In this study, the antioxidant and anti-aging activities of a polyphenol-rich strawberry extract were evaluated using human dermal fibroblasts exposed to H2O2. Firstly, the phenol and flavonoid contents of strawberry extract were studied, as well as the antioxidant capacity. HPLC-DAD analysis was performed to determine the vitamin C and β-carotene concentration, while HPLC-DAD/ESI-MS analysis was used for anthocyanin identification. Strawberry extract presented a high antioxidant capacity, and a relevant concentration of vitamins and phenolics. Pelargonidin- and cyanidin-glycosides were the most representative anthocyanin components of the fruits. Fibroblasts incubated with strawberry extract and stressed with H2O2 showed an increase in cell viability, a smaller intracellular amount of ROS, and a reduction of membrane lipid peroxidation and DNA damage. Strawberry extract was also able to improve mitochondrial functionality, increasing the basal respiration of mitochondria and to promote a regenerative capacity of cells after exposure to pro-oxidant stimuli. These findings confirm that strawberries possess antioxidant properties and provide new insights into the beneficial role of strawberry bioactive compounds on protecting skin from oxidative stress and aging. | en_EN |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | es_ES |
dc.subject | Strawberry | es_ES |
dc.subject | Anthocyanin | es_ES |
dc.subject | ROS | es_ES |
dc.subject | Lipid peroxidation | es_ES |
dc.subject | DNA damage | es_ES |
dc.subject | Fibroblasts | es_ES |
dc.title | Polyphenol-Rich Strawberry Extract Protects Human Dermal Fibroblasts against Hydrogen Peroxide Oxidative Damage and Improves Mitochondrial Functionality | en_EN |
dc.type | journal article | en_EN |
dc.rights.accessRights | open access | en_EN |
dc.identifier.doi | 10.3390/molecules19067798 | |