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dc.contributor.authorOliver, Jaime Antonio
dc.contributor.authorOrtiz Quesada, Raúl 
dc.contributor.authorMelguizo Alonso, Consolación 
dc.contributor.authorÁlvarez, Pablo Juan
dc.contributor.authorGómez-Millán, Jaime
dc.contributor.authorPrados Salazar, José Carlos 
dc.date.accessioned2014-09-02T12:50:17Z
dc.date.available2014-09-02T12:50:17Z
dc.date.issued2014
dc.identifier.citationOliver, J.A.; et al. Prognostic impact of MGMT promoter methylation and MGMT and CD133 expression in colorectal adenocarcinoma. BMC Cancer, 14: 511 (2014). [http://hdl.handle.net/10481/32876]es_ES
dc.identifier.issn1471-2407
dc.identifier.urihttp://hdl.handle.net/10481/32876
dc.description.abstractBackground: New biomarkers are needed for the prognosis of advanced colorectal cancer, which remains incurable by conventional treatments. O6-methylguanine DNA methyltransferase (MGMT) methylation and protein expression have been related to colorectal cancer treatment failure and tumor progression. Moreover, the presence in these tumors of cancer stem cells, which are characterized by CD133 expression, has been associated with chemoresistance, radioresistance, metastasis, and local recurrence. The objective of this study was to determine the prognostic value of CD133 and MGMT and their possible interaction in colorectal cancer patients. Methods: MGMT and CD133 expression was analyzed by immunohistochemistry in 123 paraffin-embedded colorectal adenocarcinoma samples, obtaining the percentage staining and intensity. MGMT promoter methylation status was obtained by using bisulfite modification and methylation-specific PCR (MSP). These values were correlated with clinical data, including overall survival (OS), disease-free survival (DFS), tumor stage, and differentiation grade. Results: Low MGMT expression intensity was significantly correlated with shorter OS and was a prognostic factor independently of treatment and histopathological variables. High percentage of CD133 expression was significantly correlated with shorter DFS but was not an independent factor. Patients with low-intensity MGMT expression and ≥50% CD133 expression had the poorest DFS and OS outcomes. Conclusions: Our results support the hypothesis that MGMT expression may be an OS biomarker as useful as tumor stage or differentiation grade and that CD133 expression may be a predictive biomarker of DFS. Thus, MGMT and CD133 may both be useful for determining the prognosis of colorectal cancer patients and to identify those requiring more aggressive adjuvant therapies. Future studies will be necessary to determine its clinical utility.es_ES
dc.description.sponsorshipThis study was supported by FEDER, Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica (I + D + I), Instituto de Salud Carlos III (FIS) through Project no. PI11/01862 and by the Consejería de Salud de la Junta de Andalucía through Project no. PI-0338. The authors are grateful to the Biobank of the Andalusian Public Healthcare System (Granada, Spain) for invaluable assistance.es_ES
dc.language.isoenges_ES
dc.publisherBiomed Centrales_ES
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 3.0 Licensees_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es_ES
dc.subjectColorectal canceres_ES
dc.subjectMGMTes_ES
dc.subjectCD133es_ES
dc.subjectMethylation statuses_ES
dc.subjectBiomarkeres_ES
dc.subjectOverall survivales_ES
dc.subjectDisease free-survivales_ES
dc.titlePrognostic impact of MGMT promoter methylation and MGMT and CD133 expression in colorectal adenocarcinomaes_ES
dc.typejournal articlees_ES
dc.rights.accessRightsopen accesses_ES
dc.identifier.doi10.1186/1471-2407-14-511


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